Sexually Transmitted Diseases

Skip Navigation LinksHome > May 2010 - Volume 37 - Issue 5 > Human Papillomavirus 16 Integration and Risk Factors Associa...
Sexually Transmitted Diseases:
doi: 10.1097/OLQ.0b013e3181c9c23f
Original Study

Human Papillomavirus 16 Integration and Risk Factors Associated in Anal Samples of HIV-1 Infected Men

Cañadas, María-Paz BSc*†; Darwich, Laila DVM, PHD*‡; Sirera, Guillermo MD, PHD§¶; Bofill, Margarita PHD*∥; Piñol, Marta MD**; Garcia-Cuyas, Francesc MD**; Llatjos, Mariona MD††; Corbasi, Patricia BSc¶; Clotet, Bonaventura MD, PHD*§¶; Videla, Sebastián MD, PHD¶; on behalf of the HIV-HPV Can Ruti Study Group

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Abstract

Background: The integration of HPV-16 DNA into the host genome is considered an important event in the progression of premalignant cervical lesions to cervical cancer. The aim of our study was to assess the prevalence of HPV-16 integration in anal cytologic specimens of HIV-1 infected men and its association with risk factors.

Patients Methods: This cross-sectional study included 269 HIV-infected males. Detection and typing of HPV-infection was done by multiplex PCR, and integration of HPV-16 by real-time PCR.

Results: The overall anal HPV-infection prevalence was 78% (209/269), 29% (77/269) for HPV-16 infection, and 9% (25/269) for HPV-16 integration. In HPV-16 infected group, the integration prevalence represented 32% (25/77). The only risk factor associated with HPV-16 integration was the time since HIV diagnosis (OR = 1.2, 95% CI: 1.0–1.3; P = 0.010). The risk factors associated with abnormal cytology results were: HPV infection (OR = 17.8, 95% CI: 6.8–46.6), HPV-16 infection (OR = 4.6, 95% CI: 2.5–8.4), and presence of HPV-16 integrated forms (OR = 11.7, 95% CI: 1.5–93.5). Moreover, in the multivariate analysis, the HPV-16 integration continued representing the most important risk factor (OR = 20, 95% CI: 1.6–226) for anal cytologic abnormalities.

Conclusion: HPV-16 infection and its integration in anal cells were highly prevalent in HIV-infected men. The assessment of HPV-16 integration rather than HPV-infection could be a good biomarker for predicting anal precancerous lesions in HIV-positive men.

© Copyright 2010 American Sexually Transmitted Diseases Association

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