Sexually Transmitted Diseases

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Sexually Transmitted Diseases:
doi: 10.1097/01.olq.0000258307.18831.f0
Article

Performance of Focus ELISA Tests for HSV-1 and HSV-2 Antibodies Among University Students With No History of Genital Herpes

Mark, Hayley D. PhD*; Nanda, Joy P. ScD†; Roberts, Jessica MSN*; Rompalo, Anne MD‡; Melendez, Johan H. MS‡; Zenilman, Jonathan MD‡

Erratum

Erratum

The authors of “Performance of Focus ELISA Tests for HSV-1 and HSV-2 Antibodies Among University Students With No History of Genital Herpes,” which appeared in Volume 34 of Sexually Transmitted Diseases on pages 681 to 685, would like to thank Wayne Hogrefe for bringing to their attention an error in their article. In the 5th paragraph in the discussion section, the sentence that reads “In our study, 2 of the samples that tested HSV-2 positive by WB tested HSV-2 negative by both focus ELISA and inhibition assays,” the positive and negative terms are inverted. The sentence should read, “In our study, 2 of the samples that tested HSV-2 negative by WB tested HSV-2 positive by both Focus ELISA and inhibition assays.”

Sexually Transmitted Diseases. 34(10):835, October 2007.

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Abstract

Objectives: To define the performance characteristics of the Focus ELISA HSV-1 and HSV-2 assay among 100 university students.

Study Design: HSV-1 and HSV-2 Focus ELISA and Western Blot assays were performed on sera from university students who reported no history of genital herpes.

Results: HSV-2 and HSV-1 seroprevalence by Western Blot were 3.4% and 48%, respectively. In this population, the positive predictive value of the Focus HSV-2 ELISA was 37.5%, the sensitivity was 100%, and specificity was 94.1%. The PPV of the Focus HSV-1 ELISA was 96.7%, the sensitivity was 69.0%, and the specificity was 97.8%.

Conclusions: In this low-prevalence population, the positive predictive value of the Focus HSV-2 ELISA test was low. This finding, together with those reported elsewhere, indicates that caution is warranted when recommending HSV screening in low-prevalence or heterogeneous populations. Consideration should be given to raising the cutoff index value for defining a positive test result.

© Copyright 2007 American Sexually Transmitted Diseases Association

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