Sexually Transmitted Diseases

Skip Navigation LinksHome > July 2007 - Volume 34 - Issue 7 > The Molecular Diagnosis of Lymphogranuloma Venereum: Evaluat...
Sexually Transmitted Diseases:
doi: 10.1097/01.olq.0000245957.02939.ea
Article

The Molecular Diagnosis of Lymphogranuloma Venereum: Evaluation of a Real-Time Multiplex Polymerase Chain Reaction Test Using Rectal and Urethral Specimens

Chen, Cheng-Yen*; Chi, Kai-Hua*; Alexander, Sarah†; Martin, Iona M. C.†; Liu, Hsi*; Ison, Cathy A.†; Ballard, Ronald C.*

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Abstract

Objectives: The objectives of this study were to evaluate the use of a real-time multiplex polymerase chain reaction (M-PCR) assay to differentiate between trachoma and lymphogranuloma venereum (LGV) biovars of Chlamydia trachomatis and to validate its performance with the conventional genotyping method.

Study: Swab specimens from 115 patients with anorectal symptoms or syndromes associated with LGV were tested by a real-time M-PCR assay and the results compared with the PCR-based restriction fragment length polymorphism analysis of the major outer membrane protein gene (omp1).

Results: A high agreement of 96.5% (111 of 115 specimens) was found between the real-time M-PCR testing and the standard genotyping method for the detection of C. trachomatis DNA (κ value, 0.945, P <0.00001). Both methods identified 53 LGV, 32 non-LGV C. trachomatis, and 26 negative specimens.

Conclusions: The real-time M-PCR assay simultaneously detects and differentiates LGV from non-LGV strains using swab specimens. This assay offers a relatively rapid and sensitive alternative for the diagnosis of LGV infection and is a useful tool for screening and for outbreak investigations.

© Copyright 2007 American Sexually Transmitted Diseases Association

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