Sexually Transmitted Diseases

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Sexually Transmitted Diseases:
doi: 10.1097/01.olq.0000218871.89901.61
Article

Development of a Nonhuman Primate Model for Trichomonas vaginalis Infection

Patton, Dorothy L. PhD*; Sweeney, Yvonne T. Cosgrove BA*; Agnew, Kathy J. BS*; Balkus, Jennifer E. MPH*; Rabe, Lorna K. BS†; Hillier, Sharon L. PhD†

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Abstract

Objective: Trichomoniasis, caused by Trichomonas vaginalis, is a prevalent sexually transmitted infection associated with increased risk of HIV infection. An animal model of T. vaginalis infection would enable scientists to further investigate trichomoniasis.

Study Design: Seven macaques (4 test vs. 3 control) were enrolled in a 2-week pilot study. Eight additional animals participated in a 2-arm (T. vaginalis vs. sham inoculated) crossover study lasting 5 weeks before treatment. In all, 12 Macaca nemestrina monkeys were challenged with a single intravaginal inoculation of 6.6 to 7.1 × 105 trichomonads (ATCC 50148). Vaginal culture (InPouch TV), colposcopy, microbiology, pH, and cervical cytokines were assessed at baseline, day 2, and weekly thereafter.

Results: Ten of 12 challenged animals tested positive for trichomoniasis for 2 weeks or longer. One animal tested positive on days 2 and 7 but negative thereafter. Only one animal was not infected. Oral metronidazole treatment (35 mg/kg per day for 3 days) resolved infection in all animals. Trichomoniasis infection did not lead to shifts in vaginal microbiology or pH.

Conclusions: A single T. vaginalis inoculation results in persistent infection in the pigtailed macaque.

© Copyright 2006 American Sexually Transmitted Diseases Association

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