Despite the progressive increase of antimicrobial resistance of Neisseria gonorrhoeae worldwide, there are limited reports of alternative remedies from plants.
The aim of the current study was to screen 3 plants, Ocimum sanctum, Drynaria quercifolia, and Annona squamosa, for activity against Neisseria gonorrhoeae.
By disc diffusion method, extracts of these 3 plants were screened for activity against Neisseria gonorrhoeae; their antimicrobial activity was calculated as percentage inhibition and compared with penicillin and ciprofloxacin.
The extracts of all 3 plants caused inhibition of Neisseria gonorrhoeae clinical isolates and World Health Organization (WHO) strains, more so than the multidrug resistant Neisseria gonorrhoeae.
Neisseria gonorrhoeae clinical isolates and WHO strains were sensitive to extracts of Ocimum sanctum, Drynaria quercifolia, and Annona squamosa. This motivates us to isolate the active component/second from the 3 plants.
Screening of extracts of Ocimum sanctum, Drynaria quercifolia, and Annona squamosa revealed their inhibitory activity towards Neisseria gonorrhoeae, more so than multidrug resistant strains.
From the * Dr. B.R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India; and the † Regional STD Teaching, Training, and Research Centre, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India.
One of the authors, Poonam Shokeen, is indebted to CSIR, New Delhi, for a Junior Research Fellowship. The authors thank Dr. J. Tapsall, Neisseria Reference Laboratory, Prince of Wales Hospital, Sydney, Australia, for supplying low-potency antibiotic discs and World Health Organization strains; Dr. S. Hegde, St. Aloysius College, Mangalore, India, for providing the leaves and rhizomes of Drynaria quercifolia; Dr. S. Chatterjee, Indian Herbs Research and Supply Company Limited, Saharanpur, India, for providing the leaves of Ocimum sanctum; and acknowledge Mrs. Leelamma Peter, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India, for excellent technical assistance. The authors also acknowledge Ajeet Narayan, Institute of Genomics and Integrative Biology, Delhi, India, for preparing the extracts of Drynaria quercifolia, Rajesh Kumar Gupta for preparing the extracts of Annona squamosa, and Dr. Uma Choudhary for invaluable suggestions and kind support.
Correspondence: Vibha Tandon, PhD, Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110007, India. E-mail: firstname.lastname@example.org.
Received for publication May 26, 2004, and accepted November 22, 2004.