Sexually Transmitted Diseases

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Article

Entry of Inflammatory Cells Into the Mouse Vagina Following Application of Candidate Microbicides: Comparison of Detergent-Based and Sulfated Polymer–Based Agents

MILLIGAN, GREGG N. PhD*; DUDLEY, KRISTEN L. BSc†; BOURNE, NIGEL PhD*; REECE, ALISA†; STANBERRY, LAWRENCE R. MD, PhD*

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Abstract

Background: Because topical microbicides designed to prevent the spread of sexually transmitted diseases may be applied frequently, it is important to ensure product safety as well as efficacy. A murine model was developed to test for induction of inflammatory responses following application of candidate microbicides.

Goal: A comparison was made of the induction of inflammation following vaginal application of detergent-based and sulfated polymer–based microbicides.

Study Design: Vaginal leukocytes were collected, identified, and quantified following microbicide application to detect the entry of inflammatory leukocytes into the vaginal lumen.

Results: Large numbers of neutrophils and macrophages entered the vaginal lumen following a single application of detergent-based microbicides. No significant increase in vaginal leukocytes was detected following a single or repeated application of sulfated polymer–based microbicides.

Conclusion: Application of sulfated polymer–based microbicides was less likely to result in inflammatory responses than was application of detergent-based compounds. This murine model should prove useful as part of a screening process to prioritize candidate microbicides before clinical trial.

© Copyright 2002 American Sexually Transmitted Diseases Association

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