You could be reading the full-text of this article now if you...

If you have access to this article through your institution,
you can view this article in

Histological Study of Chordoma Origin From Fetal Notochordal Cell Rests

Shen, Jun MD, PhD*; Shi, Qin PhD; Lu, Jian MD; Wang, Dong-Lai MD, PhD*; Zou, Tian-Ming MD*; Yang, Hui-Lin MD, PhD; Zhu, Guo-Qing MD*

Spine:
doi: 10.1097/BRS.0000000000000010
Basic Science
Abstract

Study Design. The histological comparative study was performed on chordoma and notochordal cell rests (NCRs).

Objective. To understand the histological similarity and homology of chordoma and NCRs, further supplying direct evidence of chordoma origin from NCRs.

Summary of Background Data. Although many studies supported the hypothesis that chordoma arise from NCRs, there has been little direct evidence reported to date. Of the base of our previous study, we conducted a comparative histological study among NCRs coexisting in chordoma, fetal NCRs, and chordoma tumor components.

Methods. Thirty fetal nucleus pulposus and 46 chordoma specimens were harvested, and classic chordoma tumor markers and brachyury expression levels were investigated through immunohistochemical method.

Results. The fetal NCRs existed in the form of clusters in the center of nucleus pulposus <36 gestational weeks; NCRs coexisting in chordoma specimens consisted of packed cells without extracellular myxoid matrix. Both the above-mentioned NCRs as well as chordoma tumor components showed high sensitivity for classic chordoma tumor makers (epithelial membrane antigen, AE1/AE3, CAM5.2, vimentin, S-100); both kinds of NCRs showed completely negative expression for brachyury, whereas chordoma tumor components demonstrated 100% positivity.

Conclusion. Our study results supported histological similarity and homology of NCRs coexisting in chordoma and in fetal nucleus pulposus. Brachyury activation probably takes an important role in chordoma tumoregenesis.

Level of Evidence: N/A

In Brief

Little direct evidence was proposed about the chordoma origin. Although no difference was found in classic chordoma markers, 2 notochordal cell rests in fetus and coexisting in chordoma showed complete negative expression for brachyury, whereas chordoma tumor components demonstrated 100% positivity. Brachyury activation probably takes an important role in tumoregenesis.

Author Information

*Department of Orthopaedic Surgery, Suzhou Municipal Hospital, Affiliated Suzhou Hospital, Nanjing Medical University, Suzhou, Jiangsu Province, China; and

Department of Orthopaedic Surgery, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.

Address correspondence and reprint requests to Hui-Lin Yang, MD, PhD, Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, 188, Shi Zi Road, Suzhou, Jiangsu Province, China 215006; E-mail: szshenjun@yeah.net

The manuscript submitted does not contain information about medical device(s)/drug(s).

The manuscript submitted does not contain information about medical device(s)/drug(s).

Jiangsu Province's Key Medical center (grant ZX200608) and the National Nature Science Foundation of China (grant 30672140) funds were received in support of this work.

No relevant financial activities outside the submitted work.

© 2013 by Lippincott Williams & Wilkins