Skip Navigation LinksHome > September 15, 2013 - Volume 38 - Issue 20 > Feasibility of In Vivo Quantitative Magnetic Resonance Imagi...
doi: 10.1097/BRS.0b013e31829ef69c
Basic Science

Feasibility of In Vivo Quantitative Magnetic Resonance Imaging With Diffusion Weighted Imaging, T2-Weighted Relaxometry, and Diffusion Tensor Imaging in a Clinical 3 Tesla Magnetic Resonance Scanner for the Acute Traumatic Spinal Cord Injury of Rats: Technical Note

Mondragon-Lozano, Rodrigo MSc*; Diaz-Ruiz, Araceli PhD; Ríos, Camilo PhD†,‡; Olayo Gonzalez, Roberto PhD§; Favila, Rafael MSc; Salgado-Ceballos, Hermelinda PhD; Roldan-Valadez, Ernesto MD, MSc**

Collapse Box


Study Design. Prospective longitudinal study.

Objective. To verify the feasibility of performing in vivo quantitative magnetic resonance imaging evaluation of moderate traumatic spinal cord injury (SCI) in rats using a clinical 3T scanner.

Summary of Background Data. Animal models of human diseases are essential for translational medicine. Potential treatments of SCI are evaluated in 2 ways: anatomical and functional. Advanced magnetic resonance sequences allow a noninvasive assessment of the spinal cord depicting both. This study describes and validates a very reproducible, feasible, affordable, and reliable method, designed to be applied in commercial 3T equipment, using a novel stereotactic device for spinal cord, leading to a readily available assessment of the progression of damage generated after traumatic SCI in rats.

Methods. Four Long-Evans female rats were injured with a New York University weight-drop device to produce the SCI by contusion at thoracic level 10. All animals were placed in a fixation system, using a commercial wrist antenna to obtain magnetic resonance imaging data of the relaxometry time, apparent diffusion coefficient, and fractional anisotropy. Three sets of data obtained before SCI and 1 and 4 weeks after injury were compared.

Results. The data showed a progressive decline in fractional anisotropy measurements after SCI comparing baseline versus the 1-week period (P < 0.001) and baseline versus the 4-week period (P < 0.019), with a significant progressive increase in apparent diffusion coefficient values and T2 after SCI only in the baseline versus the 4-week period (P < 0.045 and P < 0.024, respectively).

Conclusion. Our results helped us to validate a novel method to acquire highly reproducible and reliable quantitative biomarkers of traumatic SCI in vivo by using a 3T clinical MR scanner coupled with a novel stereotactic device for rats.

Level of Evidence: N/A

© 2013 by Lippincott Williams & Wilkins

Follow Us!



Article Tools


Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.