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Phosphorus and Nitrogen Fertilization Effect on Phosphorus Uptake and Phosphatase Activity in Ryegrass and Tall Fescue Grown in a Chilean Andisol

Paredes, Cecilia1; Menezes-Blackburn, Daniel1; Cartes, Paula2; Gianfreda, Liliana3; Luz Mora, María2

doi: 10.1097/SS.0b013e3182147fd3
Technical Article

A series of short-term experiments were carried out to assess the effect of phosphorus (P) and nitrogen (ammonium [NH4+-N] or nitrate [NO3-N]) fertilization on P uptake and phosphatase activity in ryegrass and tall fescue cultivated under greenhouse conditions. Ryegrass or tall fescue plants were grown in an acidic Andisol in the presence or absence of P and increasing doses of NO3-N or NH4+-N fertilizers. At the end of the experiment, soil phosphatase activity (P-aseRhiz), pH, and Olsen-P were determined in the rhizosphere soil. Plant biomass, P uptake, and root surface phosphatase (P-aseRoot) were also assayed for both plant species. Furthermore, soil incubation experiments at increasing doses of P, NO3-N, or NH4+-N were performed to evaluate the fertilizer effect on soil phosphatase activity (P-aseBulk) and microbial biomass carbon in the bulk soil. In the absence of plants, P-aseBulk was inhibited and microbial biomass carbon was raised at increasing P supply levels. In the greenhouse experiments, P uptake by tall fescue was about 67% higher than that of ryegrass at low soil P availability, which suggests that tall fescue was less sensitive to P deficiency than ryegrass. For both plant species, P-aseRhiz did not vary as a consequence of P addition. On the other hand, fertilization with the highest NH4+-N dose strongly decreased soil pH and shoot P content, as well as it increased P-aseRoot activity. This fact denotes that P-aseRoot behaved as a strategic response parameter to P stress with insufficient impact on plant P nutrition in both plant species.

1Doctorate in Science of Natural Resources, Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile. Prof. María Luz Mora is corresponding author. E-mail: mariluz@ufro.cl

2Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Avenida Francisco Salazar 01145, Temuco, Chile.

3Dipartimento di Scienze del Suolo, della Pianta, dell'Ambiente e delle Produzioni Animali, Università di Napoli Federico II, Portici, Italy.

Received July 23, 2010.

Accepted for publication February 7, 2011.

© 2011 Lippincott Williams & Wilkins, Inc.