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Approaches for molecular identification and typing of the Cryptococcus species complex: an update

Feng, Xiaoboa; Yao, Zhironga; Liao, Wanqingb

Reviews in Medical Microbiology: January 2013 - Volume 24 - Issue 1 - p 1–6
doi: 10.1097/MRM.0b013e32835736c1
Original Articles

The Cryptococcus species complex is composed of two common pathogenic species, namely Cryptococcus neoformans and Cryptococcus gattii, which cause systemic infections in both immunocompromised and immunocompetent individuals. Both species have a bipolar mating system, with mating type (MATα) being predominant in clinical and environmental isolates. On the basis of genetic variation, this species complex is divided into eight major genotypes, which show differences in epidemiology, biology, virulence and antifungal susceptibility. Within each major genotype, more diverse subgroups have been discovered and could be related to specific pathogenic attributes. This review will discuss the methods that have been developed in recent years to characterize the MATs, genotypes, varieties, species and serotypes established previously in this microorganism.

aMedical Mycology Laboratory, Xinhua Hospital, Shanghai Jiaotong University School of Medicine

bShanghai Key Laboratory of Molecular Medical Mycology, Shanghai Institute of Medical Mycology, Changzheng Hospital, Second Military Medical University, Shanghai, People's Republic of China.

Correspondence to Zhirong Yao, Medical Mycology Laboratory, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai 200092, People's Republic of China. Tel: +86 02125078999 ext. 6428; e-mail:

Received 7 May, 2012

Accepted 20 June, 2012

© 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins