Purpose: To evaluate the selectivity and strength of intraoperative trypan blue staining during removal of epiretinal membranes (ERMs) and the internal limiting membrane.
Methods: Based on intraoperative videos, 51 consecutive chromovitrectomies in 51 patients with macular holes, macular pucker, vitreomacular traction syndromes, or persistent macular edema were retrospectively studied. Fifteen subjects underwent trypan blue, 14 indocyanine green, and 22 brilliant blue G chromovitrectomy. The main outcome measure was the color contrast between stained internal limiting membrane or ERM and the underlying unstained tissue by means of objective, quantitative, semiautomated chromaticity difference measurements.
Results: Trypan blue stains both ERM and the internal limiting membrane (average chromaticity scores 8.51 and 7.09, respectively; P = 0.48). Internal limiting membrane chromaticity scores were similar for trypan blue (7.09) and brilliant blue G (6.81; P = 0.71) but clearly higher for indocyanine green (15.81; P = 2.45 × 10−5).
Conclusion: Under the premises of our study, trypan blue stains both ERM and the internal limiting membrane. Trypan blue's staining capacity of the internal limiting membrane is similar to that of brilliant blue G but significantly inferior compared with indocyanine green. Trypan blue, thus, represents a useful vital dye for chromovitrectomy, particularly in the presence of ERM, where it allows a sequential approach.
Objective, quantitative, video-based, semiautomated analyses demonstrate that trypan blue stains both epiretinal membrane and the internal limiting membrane, with a slight preference for epiretinal membrane. Affinity of trypan blue to the internal limiting membrane is similar to that of brilliant blue G but significantly inferior compared with indocyanine green.
*Department of Ophthalmology, University Hospital Basel, Basel, Switzerland
†University Eye Hospital of the Ludwig-Maximilians University, Munich, Germany
‡Department of Ophthalmology and Optometry, Linz General Hospital, Linz, Austria
§Department of Ophthalmology, Medical University Graz, Graz, Austria
¶Medical Image Analysis Center, University of Basel, Basel, Switzerland.
Reprint requests: Paul B. Henrich, MD, Department of Ophthalmology, University of Basel Eye Hospital, PO Box, CH-4012 Basel, Switzerland; e-mail: Henrichp@uhbs.ch
The authors declare no conflict of interest.