Schizophrenia, the most common major psychiatric disorder (or group of disorders), entails severe decline of higher functions, principally with alterations in cognitive functioning and reality perception. Both genetic and environmental factors are involved in its pathogenesis; however, its genetic background still needs to be clarified. The objective of the study was to reveal genetic markers associated with schizophrenia in the Bulgarian population.
We have conducted a genome-wide association study using 554 496 single nucleotide polymorphisms (SNPs) in 188 affected and 376 unaffected Bulgarian individuals. Subsequently, the 100 candidate SNPs that revealed the smallest P-values were further evaluated in an additional set of 99 case and 328 control samples.
We found a significant association between schizophrenia and the intronic SNP rs7527939 in the HHAT gene (P-value of 6.49×10−9 with an odds ratio of 2.63, 95% confidence interval of 1.89–3.66). We also genotyped additional SNPs within a 58-kb linkage disequilibrium block surrounding the landmark SNP.
We suggest rs7527939 to be the strongest indicator of susceptibility to schizophrenia in the Bulgarian population within the HHAT locus.
Supplemental Digital Content is available in the text.
aLaboratory for International Alliance
bLaboratory for Pharmacogenetics
cLaboratory for Genotyping Development, RIKEN Center for Genomic Medicine, Yokohama City, Kanagawa
dLaboratory of Statistical Analysis, RIKEN Center for Genomic Medicine
eLaboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
fDepartment of Medical Genetics, Medical Faculty
gDepartment of Psychiatry, Aleksandrovska Hospital, Medical University, Sofia
hState Psychiatric Hospital “St. Ivan Rilski” – Ministry of Health of Republic of Bulgaria, Novi Iskar
iDepartment of Psychiatry, Dr Georgi Kisiov Hospital, Radnevo, Bulgaria
jDepartment of Psychological Medicine, Cardiff University, School of Medicine, Henry Wellcome Building, Heath Park, Cardiff, UK
Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Website (http://www.psychgenetics.com).
Correspondence to Draga I. Toncheva, MD, PhD, DSci, Department of Medical Genetics, Medical Faculty, Medical University Sofia, 1431Sofia, Bulgaria Tel/fax: +359 2 952 03 57; e-mail: firstname.lastname@example.org
Received November 8, 2011
Accepted May 3, 2012