Psychiatric Genetics

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Psychiatric Genetics:
doi: 10.1097/YPG.0b013e328353ae66
Brief Reports

BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder

Gao, Yonglina; Galante, Mathewa; El-Mallakh, Jamesa; Nurnberger, John I. Jrc; Delamere, Nicholas A.d; Lei, Zhenminb; El-Mallakh, Rif S.a; BiGS Consortium

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Objective: To determine whether single nucleotide polymorphisms (SNPs) of the brain-derived neurotrophic factor (BDNF) that have been associated with bipolar illness are associated with physiological dysfunction.

Methods: Lymphoblastoid cell lines (n=30) obtained from bipolar I individuals carrying zero, one, or two copies of a BDNF SNP associated with bipolar illness (rs12273363) were utilized.

Results: Proapoptotic stressors of serum deprivation alone, or serum deprivation combined with the sodium ionophore, monensin, did not alter intracellular proBDNF. Monensin treatment increased mature-BDNF (mBDNF) protein levels (P<0.05). There were no differences related to the presence of SNP or copy number.

Conclusion: rs12273363 does not appear to have functional consequences that would involve its role in bipolar illness.

© 2012 Lippincott Williams & Wilkins, Inc.


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