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BDNF expression in lymphoblastoid cell lines carrying BDNF SNPs associated with bipolar disorder

Gao, Yonglina; Galante, Mathewa; El-Mallakh, Jamesa; Nurnberger, John I. Jrc; Delamere, Nicholas A.d; Lei, Zhenminb; El-Mallakh, Rif S.a; BiGS Consortium

Psychiatric Genetics:
doi: 10.1097/YPG.0b013e328353ae66
Brief Reports

Objective: To determine whether single nucleotide polymorphisms (SNPs) of the brain-derived neurotrophic factor (BDNF) that have been associated with bipolar illness are associated with physiological dysfunction.

Methods: Lymphoblastoid cell lines (n=30) obtained from bipolar I individuals carrying zero, one, or two copies of a BDNF SNP associated with bipolar illness (rs12273363) were utilized.

Results: Proapoptotic stressors of serum deprivation alone, or serum deprivation combined with the sodium ionophore, monensin, did not alter intracellular proBDNF. Monensin treatment increased mature-BDNF (mBDNF) protein levels (P<0.05). There were no differences related to the presence of SNP or copy number.

Conclusion: rs12273363 does not appear to have functional consequences that would involve its role in bipolar illness.

Author Information

aMood Disorder Research Program, Department of Psychiatry and Behavioral Science

bDepartment of Obstetrics and Gynecology, School of Medicine, University of Louisville, Louisville, Kentucky

cDepartment of Psychiatry, Indiana University School of Medicine, Indianapolis, Indiana

dDepartment of Physiology, University of Arizona Health Sciences Center, Tucson, Arizona, USA

Correspondence to Rif S. El-Mallakh, MD, Mood Disorder Research Program, Department of Psychiatry and Behavioral Science, School of Medicine, University of Louisville, Louisville, KY 40292, USA Tel: +1 502 852 5866; fax: +1 502 852 5098; e-mail:

Received June 6, 2011

Accepted January 12, 2012

© 2012 Lippincott Williams & Wilkins, Inc.