Objective: To determine whether single nucleotide polymorphisms (SNPs) of the brain-derived neurotrophic factor (BDNF) that have been associated with bipolar illness are associated with physiological dysfunction.
Methods: Lymphoblastoid cell lines (n=30) obtained from bipolar I individuals carrying zero, one, or two copies of a BDNF SNP associated with bipolar illness (rs12273363) were utilized.
Results: Proapoptotic stressors of serum deprivation alone, or serum deprivation combined with the sodium ionophore, monensin, did not alter intracellular proBDNF. Monensin treatment increased mature-BDNF (mBDNF) protein levels (P<0.05). There were no differences related to the presence of SNP or copy number.
Conclusion: rs12273363 does not appear to have functional consequences that would involve its role in bipolar illness.
aMood Disorder Research Program, Department of Psychiatry and Behavioral Science
bDepartment of Obstetrics and Gynecology, School of Medicine, University of Louisville, Louisville, Kentucky
cDepartment of Psychiatry, Indiana University School of Medicine, Indianapolis, Indiana
dDepartment of Physiology, University of Arizona Health Sciences Center, Tucson, Arizona, USA
Correspondence to Rif S. El-Mallakh, MD, Mood Disorder Research Program, Department of Psychiatry and Behavioral Science, School of Medicine, University of Louisville, Louisville, KY 40292, USA Tel: +1 502 852 5866; fax: +1 502 852 5098; e-mail: firstname.lastname@example.org
Received June 6, 2011
Accepted January 12, 2012