Background: Although platelet-rich plasma (PRP) is nowadays a common method in various medical fields, including cosmetic surgery or dermatology, the expensiveness of the kit for processing is still a hurdle.
Methods: A new unique economic method for preparing PRP was reported. The method consists in a simple modification of a disposable 5-mL syringe that allows insertion into a common centrifuge and positioning of the syringe on the centrifuge so the PRP separates next to the tip of the syringe. Platelet-derived growth factor BB in PRP was measured under anticoagulant dextrose solution A (ACD-A) or heparin as anticoagulant and with or without prostaglandin E1 (PGE1) as a platelet aggregation suppressant.
Results: The new method successfully created PRP with high platelet-derived growth factor BB in all conditions, and the highest value was obtained by using ACD-A and PGE1.
Conclusions: The new method is useful, and the use of ACD-A and PGE1 is the most recommended.
From the Tsurumai Kouen Clinic, Nagoya, Japan.
Received for publication December 26, 2013; accepted April 7, 2014.
Disclosure: The authors have no financial interest to declare in relation to the content of this article. The Article Processing Charge was paid for by the authors.
Mototsugu Fukaya, MD, Tsurumai Kouen Clinic, 5-20-6 Chiyoda, Nakaku, Nagoya 460-0012, Japan, E-mail: firstname.lastname@example.org
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