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Point of Care: The Journal of Near-Patient Testing & Technology:
doi: 10.1097/POC.0000000000000016
Original Articles

The Effects of Various Flow Velocities on the Sensitivity of an Enzyme-Linked Immunosorbent Assay in a Fluidic Allergy Diagnostic Device

Drescher, Philipp Dipl-Ing*; Dangers, Marc PhD; Rübenhagen, René PhD; Seitz, Hermann Dr-Ing*

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Abstract

A novel point-of-care allergy diagnostic device was used to investigate the influence of flow velocities on the sensitivity of an enzyme-linked immunosorbent assay (ELISA). Various flow rates were applied in a test setup, and the reaction sensitivity of the ELISA as well as fluidic properties within the point-of-care device such as pressure, delivery volume, and air bubble formation was analyzed. The results show that a velocity dependency seems to exist and that there is a peak in the sensitivity of the ELISA between 0.03 and 0.06 m/s in this configuration. These results can be used in future ELISAs to further improve its effectiveness and efficiency.

Copyright © 2014 by Lippincott Williams & Wilkins

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