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A Novel Technology for 5-Part Differentiation of Leukocytes Point-of-Care

Lindberg, Stellan MSc*; Jönsson, Inger BSc; Nilsson, Magnus BSc; Johnsson, Elin BSc*; Jonasson-Bjäräng, Tomas MSc*

Point of Care: The Journal of Near-Patient Testing & Technology: June 2014 - Volume 13 - Issue 2 - p 27–30
doi: 10.1097/POC.0000000000000015
Original Articles

Abstract: A new point-of-care hematology system has recently been introduced by HemoCue. Whole blood is sampled directly from a fingerstick sample or venous blood tube into a microcuvette. The measurement starts automatically when the microcuvette is inserted into the analyzer. The novel system uses state-of-the-art imaging technology to count the white blood cells (WBCs) and perform a 5-part differentiation. The cells are classified into neutrophils, lymphocytes, monocytes, eosinophils, basophils, pathological WBCs (blasts and immature granulocytes), and others.

The HemoCue WBC DIFF gave excellent correlation to 3 different cell counters with a correlation coefficient (r) between 0.94 and 0.997 for total WBCs, neutrophils, and lymphocytes. Only a very small bias (<0.24 × 109/L) could be observed on those cell types. A point-of-care WBC count including a 5-part differential will increase the availability at the point-of-care of already well-established and frequently used laboratory tests. Rapid and easy access will assist physicians in making direct and more evidence-based decisions in a number of clinical conditions.

From the *HemoCue AB, Ängelholm, and †Department of Clinical Chemistry, Central Hospital, Växjö, Sweden.

Reprints: Stellan Lindberg, MSc, HemoCue AB, Box 1204, SE-262 23, Ängelholm, Sweden. E-mail:

E. Johnsson, T. Jonasson-Bjäräng, and S. Lindberg are employed by HemoCue AB, Sweden.

The remaining authors declare no conflicts of interest.

HemoCue AB supported this study with instruments and microcuvettes during the evaluation. Each hospital and physicians’ offices were reimbursed for all extra ordinary costs involved in this work. No extra financial grants or gifts were given.

© 2014 by Lippincott Williams & Wilkins