Skip Navigation LinksHome > April 2012 - Volume 129 - Issue 4 > Reply: TallyHo Diabetic Phenotype Limited to Male Mice: Fe...
Plastic & Reconstructive Surgery:
doi: 10.1097/PRS.0b013e318245eb24
Letters

Reply: TallyHo Diabetic Phenotype Limited to Male Mice: Female Mice Provide Obese, Nondiabetic Mouse Model

Buck, Donald W. II M.D.; Mustoe, Thomas A. M.D.

Free Access
Article Outline
Collapse Box

Author Information

Division of Plastic and Reconstructive Surgery, Northwestern University, Feinberg School of Medicine, Chicago, Ill.

Correspondence to Dr. Buck, Division of Plastic and Reconstructive Surgery, Northwestern University, Feinberg School of Medicine, 675 North St. Clair Street, Galter 19-250, Chicago, Ill. 60611

Back to Top | Article Outline

Sir:

We appreciate Dr. Wagner's interest in our article and her comments regarding sex differences between the TallyHo/JnJ mice. In our recent article, “The TallyHo Polygenic Mouse Model of Diabetes: Implications in Wound Healing,”1 we used only male TallyHo mice. Sex dimorphism for the diabetic phenotype is commonly observed in murine models of type 2 diabetes, and the TallyHo strain is no exception. In 2006, Kim et al. published an extensive phenotypic analysis of the TallyHo strain, demonstrating that male mice developed diabetes whereas female mice did not.2 It has been postulated that the protection of diabetes in female mice is at least partially attributable to estrogen-dependent increases in insulin receptors in the hepatocyte membranes and low hepatic estrogen sulfotransferase activity, which prevent insulin resistance and virilization of liver metabolism, respectively.3,4 Although female TallyHo mice do not express an overt diabetic phenotype, they are more obese, with mild hyperinsulinemia, mild hypertriglyceridemia, and pancreatic islet cell hypertrophy when compared with nondiabetic control mice. As a result, they do represent an obese, nondiabetic murine model.

Figure. No caption a...
Figure. No caption a...
Image Tools

Although there is a paucity of literature specifically defining blood glucose levels required for the diagnosis of diabetes in mice, there is substantial evidence regarding the development of the cellular derangements characteristic of diabetes and the correlative blood glucose levels at which they occur.2 The progression of type 2 diabetes is characterized by impaired glucose tolerance, insulin resistance, and finally failure of insulin secretion. Simplistically, these events could be characterized by hyperglycemia, hyperinsulinemia, and pancreatic islet cell hypertrophy and/or atrophy. The male TallyHo mouse begins this progression at 4 weeks of age and, by the eighth week of life, exhibits significantly impaired glucose tolerance, insulin resistance, and islet cell hypertrophy, consistent with diabetes. The blood glucose levels at 8 weeks are above the 200-mg/dl range and continue to rise, peaking in the 300- to 400-mg/dl range by 14 weeks of life.1

Donald W. Buck, II, M.D.

Thomas A. Mustoe, M.D.

Division of Plastic and Reconstructive Surgery, Northwestern University, Feinberg School of Medicine, Chicago, Ill.

Back to Top | Article Outline

REFERENCES

1. Buck DW II, Jin DP, Geringer M, Hong SJ, Galiano RD, Mustoe TA. The TallyHo polygenic mouse model of diabetes: Implications in wound healing. Plast Reconstr Surg. 2011;128:427e–437e. Epublished ahead of print July 5, 2011.

2. Kim JH, Stewart TP, Soltani-Bejnood M, et al.. Phenotypic characterization of polygenic type 2 diabetes in TALLYHO/JngJ mice. J Endocrinol. 2006;191:437–446.

3. Leiter EH, Chapman HD. Obesity-induced diabetes (diabesity) in C57BL/KsJ mice produces aberrant trans-regulation of sex steroid sulfotransferase genes. J Clin Invest. 1994;93:2007–2013.

4. Krakower GR, Meier DA, Kissebah AH. Female sex hormones, perinatal, and peripubertal androgenization on hepatocyte insulin dynamics in rats. Am J Physiol. 1993;264:E342–E347.

Back to Top | Article Outline
GUIDELINES

Letters to the Editor, discussing material recently published in the Journal, are welcome. They will have the best chance of acceptance if they are received within 8 weeks of an article's publication. Letters to the Editor may be published with a response from the authors of the article being discussed. Discussions beyond the initial letter and response will not be published. Letters submitted pertaining to published Discussions of articles will not be printed. Letters to the Editor are not usually peer reviewed, but the Journal may invite replies from the authors of the original publication. All Letters are published at the discretion of the Editor.

Letters submitted should pose a specific question that clarifies a point that either was not made in the article or was unclear, and therefore a response from the corresponding author of the article is requested.

Authors will be listed in the order in which they appear in the submission. Letters should be submitted electronically via PRS' enkwell, at www.editorialmanager.com/prs/.

We reserve the right to edit Letters to meet requirements of space and format. Any financial interests relevant to the content of the correspondence must be disclosed. Submission of a Letter constitutes permission for the American Society of Plastic Surgeons and its licensees and asignees to publish it in the Journal and in any other form or medium.

The views, opinions, and conclusions expressed in the Letters to the Editor represent the personal opinions of the individual writers and not those of the publisher, the Editorial Board, or the sponsors of the Journal. Any stated views, opinions, and conclusions do not reflect the policy of any of the sponsoring organizations or of the institutions with which the writer is affiliated, and the publisher, the Editorial Board, and the sponsoring organizations assume no responsibility for the content of such correspondence.

The Journal requests that individuals submit no more than five (5) letters to Plastic and Reconstructive Surgery in a calendar year.

©2012American Society of Plastic Surgeons

Login

Article Tools

Images

Share


The Clinical Masters of PRS – Breast eBooks
4 Essential eBooks for Plastic Surgeons