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Adipose-Derived Stem Cells Exhibit Antioxidative and Antiapoptotic Properties to Rescue Ischemic Acute Kidney Injury in Rats

Shih, Yu-Chung M.D.; Lee, Pei-Ying Ph.D.; Cheng, Henrich M.D., Ph.D.; Tsai, Chi-Han; Ma, Hsu M.D., Ph.D.; Tarng, Der-Cherng M.D., Ph.D.

Plastic & Reconstructive Surgery:
doi: 10.1097/PRS.0b013e3182a806ce
Experimental: Original Articles
Abstract

Background: Acute kidney injury is a major challenge in critical care medicine, with high rates of in-hospital morbidity and mortality. Stem cell therapy has emerged as an evolving technology that could have a substantial impact on acute kidney injury outcomes in the critical care environment. Therefore, the authors investigated the therapeutic effects of adipose-derived stem cells in ischemic acute kidney injury in rats.

Methods: The authors used an ischemia-reperfusion–induced acute kidney injury rat model. The effects of rescuing acute kidney injury were assessed with regard to different adipose-derived stem cell numbers and various routes of administration compared with sham-operated and phosphate-buffered saline–treated groups.

Results: Both intrarenal arterial and intravenous administration of adipose-derived stem cells reduced blood urea nitrogen and creatinine levels, and also decreased the tubular injury score 48 hours after ischemia-reperfusion–induced acute kidney injury in a dose-dependent manner, compared with the phosphate-buffered saline–treated group. In the authors’ study, it was determined that the optimal cell number was 5 × 105. Furthermore, adipose-derived stem cell transplantation exhibited antioxidative and antiinflammatory properties to reduce apoptosis and promote proliferation of renal tubular cells.

Conclusions: An optimal number of adipose-derived stem cells administered by means of the intrarenal arterial or the intravenous route effectively rescued ischemia-reperfusion–induced acute kidney injury in rats. Antioxidative and antiapoptotic properties of adipose-derived stem cells to reduce tubular cell injury also merit recognition and further study.

Author Information

Taipei, Taiwan

From the Division of Plastic Surgery, Department of Surgery, Division of Nephrology, Department of Medicine, and Department of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital; and School of Medicine, Department and Institute of Physiology, Institute of Clinical Medicine, and Institute of Pharmacology, National Yang-Ming University.

Received for publication May 23, 2013; accepted July 1, 2013.

The first two authors contributed equally to this work.

Disclosure: None of the authors has a financial interest in any of the products or devices mentioned in this article.

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Hsu Ma, M.D., Ph.D., Division of Plastic Surgery, Department of Surgery, Taipei Veterans General Hospital, 201, Sec. 2, Shih-Pai Road, Taipei 11217, Taiwan, sma@vghtpe.gov.tw

©2013American Society of Plastic Surgeons