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The Effect of Subcutaneous Mesenchymal Stem Cell Injection on Statis Zone and Apoptosis in an Experimental Burn Model

Öksüz, Sinan M.D.; Ülkür, Ersin M.D.; Öncül, Oral M.D.; Köse, Gamze Torun Ph.D.; Küçükodac, Zafer M.D.ı; Urhan, Muammer M.D.

Plastic & Reconstructive Surgery: March 2013 - Volume 131 - Issue 3 - p 463–471
doi: 10.1097/PRS.0b013e31827c6d6f
Experimental: Original Articles

Background: In an acute burn injury the zone of stasis is initially vital but may progress to coagulation necrosis with time. In this study, salvage of the zone of stasis was aimed at by subcutaneous mesenchymal stem cell injection.

Methods: Mesenchymal stem cells were obtained from the bone marrow of Sprague-Dawley rats (n = 10). Twenty Sprague-Dawley rats received thermal injury on the back according to the previously described “comb burn” model. Thirty minutes after the burn injury, mesenchymal stem cells were injected subcutaneously to the stasis zone of the experimental group (n = 10). Animals in the control group (n = 10) were given the same amount of saline without mesenchymal stem cells. Animals in the sham group (n = 6) did not receive any thermal trauma. Seventy-two hours after the burn injury, scintigraphic examination was applied to determine average vital tissue at the stasis zone. Thereafter, skin samples were assessed by immunohistochemistry assay for apoptosis count. The blood samples drawn before and 72 hours after the burn injury were analyzed to determine systemic cytokine levels.

Results: The apoptosis count of the control group was found to be significantly higher than that of the experimental group. Vital tissue percentage of the stasis zone was significantly higher for the experimental group than for the control group. The cytokine levels did not reveal any statistically significant difference between the groups.

Conclusion: Apoptosis count and scintigraphic results of this study confirm that mesenchymal stem cell treatment has a statistically significant benefit for the survival of the stasis zone in acute burn.

Istanbul, Turkey

From the Departments of Plastic Reconstructive and Aesthetic Surgery and Burn Unit, Infectious Disease and Clinical Microbiology, Pathology, and Nuclear Medicine, Gulhane Military Medical Academy, Haydarpasa Training Hospital, and the Department of Genetics and Bioengineering, Yeditepe University.

Received for publication June 18, 2012; accepted June 26, 2012.

Oral presentation at Plastic Surgery The Meeting, in New Orleans, Louisiana, October 26 through 30, 2012.

Disclosure: The authors have no financial interest to declare in relation to the content of this article.

Sinan Öksüz, M.D.; Plastik Rekonstruktif ve Estetik Cerrahi Servisi, GATA Haydarpasa Egitim Hastanesi, Selimiye Mah. Tibbiye Cad. Uskudar, 34668 Istanbul, Turkey, sinanoksuz@gmail.com

©2013American Society of Plastic Surgeons