Background: Soft-tissue repair is currently limited by the availability of autologous tissue sources and the absence of an ideal soft-tissue replacement comparable to native adipose tissue. Extracellular matrix–based biomaterials have demonstrated great potential as instructive scaffolds for regenerative medicine, mechanically and biochemically defined by the tissue of origin. As such, the distinctive high lipid content of adipose tissue requires unique processing conditions to generate a biocompatible scaffold for soft-tissue repair.
Methods: Human adipose tissue was decellularized to obtain a matrix devoid of lipids and cells while preserving extracellular matrix architecture and bioactivity. To control degradation and volume persistence, the scaffold was cross-linked using hexamethylene diisocyanate and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. In vitro studies with human adipose-derived stem cells were used to assess cell viability and adipogenic differentiation on the biomaterial. In vivo biocompatibility and volume persistence were evaluated by subcutaneous implantation over 12 weeks in a small-animal model.
Results: The scaffold provided a biocompatible matrix supporting the growth and differentiation of adipose-derived stem cells in vitro. Cross-linking the matrix increased its resistance to enzymatic degradation. Subcutaneous implantation of the acellular adipose matrix in Sprague-Dawley rats showed minimal inflammatory reaction. Adipose tissue development and vascularization were observed in the implant, with host cells migrating into the matrix indicating the instructive potential of the matrix for guiding tissue remodeling and regeneration.
Conclusions: With its unique biological and mechanical properties, decellularized adipose extracellular matrix is a promising biomaterial scaffold that can potentially be used allogenically for the correction of soft-tissue defects.
From the Departments of Biomedical Engineering, Otolaryngology, and Plastic Surgery, The Johns Hopkins University School of Medicine.
Received for publication May 11, 2011; accepted December 19, 2011.
The first two authors contributed equally to this work.
Presented at the Annual Meeting of the Tissue Engineering and Regenerative Medicine International Society, in Orlando, Florida, December 5 through 8, 2010.
Disclosure: Iwen Wu and Drs. Nahas, Rosson, and Elisseeff are inventors on intellectual property licensed to Aegeria Soft Tissue LLC. Kelly A. Kimmerling has no commercial associations or financial disclosures to report.
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Jennifer H. Elisseeff, Ph.D.; Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Smith 5035, 400 North Broadway, Baltimore, Md. 21287, firstname.lastname@example.org