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Waveform Modulation of Negative-Pressure Wound Therapy in the Murine Model

Dastouri, Pouya M.D.; Helm, Douglas L. M.D.; Scherer, Saja S. M.D.; Pietramaggiori, Giorgio M.D.; Younan, George M.D.; Orgill, Dennis P. M.D., Ph.D.

Plastic & Reconstructive Surgery: April 2011 - Volume 127 - Issue 4 - pp 1460-1466
doi: 10.1097/PRS.0b013e31820a63cb
Experimental: Original Articles

Background: Negative-pressure wound therapy applied with a porous foam interface has been shown to accelerate granulation-tissue formation when a cyclic application mode of suction is applied, but the optimal waveform has not been determined. The authors hypothesized that changes in the suction waveform applied to wounds would modulate the biological response of granulation tissue formation.

Methods: A vacuum-assisted closure device (Kinetic Concepts, Inc., San Antonio, Texas) was applied to full-thickness wounds in 48 male diabetic mice (C57BL/KsJ-Lepr db), which were treated with six different waveforms: square waveforms of 125 mmHg of suction for 2 minutes, alternating with 50 mmHg of suction for 2 minutes, 5 minutes, or 10 minutes; triangular waveform with a 7-minute period oscillating between 50 and 125 mmHg; and static suction at 125 mmHg or static suction at 0 mmHg (occlusive dressing). Wounds were quantitatively evaluated for granulation tissue thickness as well as the number of proliferating cells and the number of blood vessels of the newly formed granulation tissue.

Results: At 7 days, the continuous and triangular waveforms induced the thickest granulation tissue, with high rates of cellular proliferation and blood vessel counts compared with square wave and occlusive dressing control wounds. Decreasing square waveform frequency significantly increased granulation tissue thickness, cellular proliferation, and blood vessel counts.

Conclusions: Waveform modulation has a significant effect on granulation tissue formation, angiogenesis, and cellular proliferation in excisional wounds in diabetic mice. The rapid change in pressure seen in our square wave model may be detrimental to granulation tissue formation.

Boston, Mass.; and Geneva, Switzerland

From the Division of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, and the Division of Plastic Surgery, Hopitaux Universitaires de Geneve.

Received for publication April 30, 2010; accepted October 7, 2010.

Presented at the American College of Surgeons Clinical Congress, in Chicago, Illinois, October 14, 2009.

Disclosure: Dr. Orgill has been a consultant and expert witness for Kinetic Concepts, Inc., San Antonio, Texas, and is a principal investigator on basic research studies on the vacuum-assisted closure device conducted through a grant to Brigham and Women's Hospital from Kinetic Concepts, Inc. The other authors have no related financial interests to disclose.

Dennis P. Orgill, M.D., Ph.D., Division of Plastic Surgery, Brigham and Women's Hospital, 75 Francis Street, Boston, Mass. 02115, dorgill@partners.org

©2011American Society of Plastic Surgeons