Pediatric Infectious Disease Journal:
Effectiveness of 2 Rotavirus Vaccines Against Rotavirus Disease in Taiwanese Infants
Chang, Wan-Chi PhD*; Yen, Catherine MD, MPH†; Wu, Fang-Tzy MS‡; Huang, Yhu-Chering MD, PhD§; Lin, Jen-Shiou MD¶; Huang, Fu-Chen MD‖; Yu, Hui-Tzu MS*; Chi, Cheng-Liang MS*; Lin, Han-Ying MS*; Tate, Jacqueline E. PhD†; Parashar, Umesh D. MBBS, MPH†; Wu, Ho-Sheng PhD‡ **; Hsiung, Chao A. PhD*
From the *Division of Biostatistics and Bioinformatics, Institute of Population Health Sciences, National Health Research Institutes, Zhunan, Taiwan; †Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA; ‡Department of Health, Research and Diagnostic Center, Centers for Disease Control, Taipei; §Division of Pediatric Infectious Diseases, Chang Gung Memorial Hospital at Linkou, and Chang Gung University College of Medicine, Taoyuan; ¶Department of Laboratory Medicine, Changhua Christian Hospital, Changhua; ‖Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung; and **School of Medical Laboratory Science and Biotechnology, Taipei Medical University, Taipei, Taiwan.
Accepted for publication October 1, 2013.
This study was financially supported by the following research grant: “Technology Project for Surveillance and Prevalence of Gastroenteritis in Taiwan” from Centers for Disease Control, Department of Health, Execute Yuan, Taiwan (supported grant: DOH98-DC-1005). The authors have no other funding or conflicts of interest to disclose.
The findings and conclusions of this report are those of the authors and do not necessarily represent the official position of the US Centers for Disease Control and Prevention. This study was approved by the Institutional Review Board at all surveillance sites and by the Taiwan CDC and Taiwan NHRI. Informed consent was obtained from parents or guardians of all participants before enrollment.
Address for correspondence: Chao A. Hsiung, PhD, Division of Biostatistics and Bioinformatics, Institute of Population Health Sciences, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County 35053, Taiwan, ROC. E-mail: firstname.lastname@example.org.
Background: Two rotavirus (RV) vaccines (Rotarix and RotaTeq) are available on the private market in Taiwan, but are not recommended for routine use. We examined RV vaccine effectiveness (VE) against severe RV acute gastroenteritis (AGE) among Taiwanese infants to inform policymakers on the potential benefits of national RV vaccine introduction.
Methods: From May 2009 to April 2011, a case-control assessment of VE against severe RV AGE was conducted at 3 hospital-based surveillance sites in Taiwan. Case-patients included children aged 8–35 months, hospitalized with laboratory-confirmed RV AGE. Controls included children age-matched within 1 month of age of the case-patient, hospitalized with RV-negative AGE or seen for non-AGE illnesses at the same hospitals. Vaccination history was confirmed through vaccination card or hospital record review. VE was calculated as (1 − odds ratio of vaccination)×100%.
Results: We enrolled 184 case-patients with RV AGE, 904 RV-negative AGE and 909 non-AGE controls. Two-dose Rotarix series VE against RV gastroenteritis hospitalization was 90.4% [95% confidence interval (CI): 70.3%, 98.1%) and 92.5% (95% CI: 77.1%, 98.5%) with RV-negative AGE and non-AGE controls, respectively. Three-dose RotaTeq series VE was 96.8% (95% CI: 82.3%, 100%) and 97.1% (95% CI: 84%, 100%) with RV-negative AGE and non-AGE controls, respectively.
Conclusions: Both vaccines provided excellent protection against severe RV AGE hospitalization. Addition of RV vaccination into Taiwan’s National Immunization Program could substantially decrease AGE hospitalizations among children <3 years. Our findings should help inform policymakers in Taiwan and other similar Asian countries when deciding whether to include RV vaccination into their national immunization programs.
Rotavirus (RV) is the leading cause of severe acute gastroenteritis (AGE) among young children worldwide, accounting for approximately 23 million outpatient visits, 2.3 million hospitalizations and almost half a million deaths annually among children <5 years of age.1–3 Before 2007, RV infection accounted for an estimated 25–43% of all AGE hospitalizations among children <5 years of age in Taiwan, with many RV hospitalizations occurring by the age of 3 years.4–6 To help mitigate this significant disease burden, in August 2006, the Department of Health in Taiwan licensed 2 orally administered, live, attenuated RV vaccines—Rotarix (GSK Biologicals, Rixensart, Belgium) and RotaTeq (Merck & Co., Inc., PA). Rotarix is a monovalent vaccine given to infants in Taiwan in 2 doses at ages 2 and 4 months; RotaTeq is a pentavalent vaccine given to infants in Taiwan in 3 doses at ages 2, 4 and 6 months. Prelicensure clinical trials of both vaccines conducted in various middle- and high-income countries, including Taiwan, demonstrated vaccine efficacy of 85–98% against severe RV AGE.7–9 However, both vaccines are available only on the private market and have not yet been recommended for routine use in Taiwanese infants.
Because the performance of vaccines in real world settings may differ from that in clinical trial settings, post-licensure evaluation of vaccine performance is crucial for monitoring and sustaining vaccination programs. Vaccine effectiveness (VE) studies from Latin America, the United States and Europe have demonstrated effectiveness of Rotarix and RotaTeq comparable with that seen in the prelicensure clinical trials.10–14 However, no studies have been published regarding VE in Asian countries that are currently using these vaccines in the private sector. Data from such studies could help these countries weigh the benefits of RV vaccine introduction into national immunization programs. In addition, the simultaneous introduction of Rotarix and RotaTeq in Taiwan provides a unique opportunity to assess the field performance of both RV vaccines in the same population. Our objective was to examine VE of both Rotarix and RotaTeq against severe RV AGE resulting in hospitalization among children in Taiwan.
Hospital-based Surveillance System
In 2004, the Taiwan Centers for Disease Control (CDC) established a sentinel surveillance system for RV AGE at 3 hospitals located in the northern, middle and southern regions of Taiwan: the Chang Gung Memorial Hospital, Linkou Branch, the Changhua Christian Hospital and the Chang Gung Memorial Hospital, Kaohsiung Branch, respectively. At each hospital, active surveillance for AGE (defined as ≥3 episodes of vomiting and/or loose stools and/or bloody/mucoid stools in a 24-hour period and with onset <14 days before hospitalization) among children <5 years of age is conducted year-round. Bulk stool specimens of cases are collected within 48 hours of admission and are tested by each hospital-based laboratory for RV by an enzyme immunosorbant assay (EIA; RIDASCREEN Rotavirus, RBiopharm AG, Germany). All specimens (both positive and negative specimens) are then sent to the Viral Enteric and Emerging Diseases Laboratory at the Taiwan CDC for confirmatory testing and further strain characterization of EIA-positive specimens by real-time reverse-transcription polymerase chain reaction (RT-PCR) and nucleotide sequencing.6,15
Matched Case-control Study
From May 2009 through April 2011, a case-control assessment of Rotarix and RotaTeq VE against severe RV gastroenteritis resulting in hospitalization was conducted at all 3 sentinel surveillance sites in collaboration with the Taiwan CDC and the Taiwan National Health Research Institutes (NHRI). This study was approved by the Institutional Review Board at all surveillance sites and by the Taiwan CDC and Taiwan NHRI. Informed consent was obtained from parents or guardians of all participants before enrollment. Case-patients were defined as children aged 8–35 months hospitalized with laboratory-confirmed RV AGE. Children 8 months of age and older were selected for enrollment to ensure that all children had reached the maximum age limit for RV vaccine administration to limit confounding by age. Case-patients were matched with 2 groups of controls. One group included children who were born within 1 month of the case-patient’s date of birth and hospitalized with RV-negative AGE. A second group included children who were born within 1 month of the case-patient’s date of birth and seen for medical care for illnesses unrelated to diarrhea in either the outpatient or inpatient setting at the same sentinel hospitals. Age matching within 1 month of the date of birth was performed to control for changing vaccine coverage over time. Information on demographics, socioeconomic indicators (ie, maternal education, number of family members in the household, family income), birth history, duration of breastfeeding, history of breastfeeding within the week before hospitalization and clinical symptoms were obtained through interview of a parent or guardian. RV vaccination history and vaccine type were confirmed through vaccination card or hospital record review. Given our sample size of children enrolled through sentinel surveillance for RV AGE and assuming an estimated vaccine coverage of 50%, this study was powered adequately to detect a VE of ≥60%.
Case-patients and controls were considered vaccinated if the vaccination date was ≥14 days before the case-patient’s date of hospital admission. Differences in characteristics between case-patients and controls were separately assessed using t-tests for ages, Wilcoxon rank-sum tests for the number of family members and duration of breastfeeding and χ2 tests for categorical variables.
Exact conditional logistic regression was used to estimate an odds ratio of vaccination in case-patients compared with their matched controls. This method was used because no cases had received a full 3-dose series of RotaTeq, which would result in a zero-cell situation leading to estimates with wide confidence intervals (CIs). Thus, exact conditional inference was applied for the unstable model, regardless of other confounders; only 1 predictor of RV vaccine type at 5 levels (1-dose Rotarix, 2-dose Rotarix, 1- or 2-dose RotaTeq, 3-dose RotaTeq and unvaccinated) was used in the final model.
VE of RV vaccine against laboratory-confirmed RV AGE resulting in hospitalization was calculated as (1 − odds ratio of vaccination) × 100%. Statistical significance was designated as a 2-tailed P-value of <0.05. Sensitivity analyses were performed to determine the impact of excluded controls without vaccination history on VE estimates. The 127 controls missing vaccination history were included in 3 models under the following assumptions: (1) all were unvaccinated; (2) all were fully vaccinated with Rotarix; and (3) all were fully vaccinated with RotaTeq. All analyses were performed using SAS statistical software (version 9·1, SAS Institute, Cary, NC).
Over the 2-year surveillance period, 1280 children 8–35 months of age hospitalized with AGE were enrolled at the 3 surveillance sentinel sites (Fig. 1); 184 (14%) tested positive for RV by EIA and were enrolled as cases. The remaining 1096 RV-negative AGE patients were enrolled as controls. Additionally, 1183 non-AGE patients were enrolled as another group of controls. The 184 case-patients were then matched with 943 RV-negative AGE controls at a ratio of up to 6 controls for each case and 997 non-AGE controls at a ratio of up to 6 controls for each case. One hundred twenty-seven controls with missing vaccination information were excluded (vaccination information was available for all case-patients). Thus, 184 case-patients, 904 RV-negative AGE controls and 909 non-AGE controls were included in the final analysis.
Case-patients significantly differed from RV-negative controls and from non-AGE controls by age, likely due to the variable number of controls per case (Table 1). The average age for case-patients was 20.5 months compared with 18.2 and 19 months for the RV-negative and non-AGE controls, respectively. Case-patients also significantly differed from both types of controls by season of enrollment; 39.1% of case-patients were enrolled during the winter season (ie, December through February), while 20.4% and 19.4% of RV-negative and non-AGE controls were enrolled, respectively. Additionally, 47.5% of mothers of case-patients had a college degree or higher compared with 63% of mothers of non-AGE controls.
RT-PCR and nucleotide sequencing of RV strains from positive stool specimens demonstrated a predominance of G1P RV in 127/184 (69%) of specimens (Fig. 2); G2P RV accounted for 23/184 (12.5%). Three of 184 (1.6%) specimens were confirmed to be EIA positive, but were untypeable by RT-PCR and nucleotide sequencing.
A 2-dose series of Rotarix had been given to 1.6% of case-patients versus 14.9% and 18.9% of RV-negative and non-AGE controls who had not received RotaTeq, respectively (P < 0.0001; Table 1). Estimated VE of a full 2-dose Rotarix series against RV gastroenteritis resulting in hospitalization was 90.4% (95% CI: 70.3%, 98.1%) and 92.5% (95% CI: 77.1%, 98.5%) with RV-negative and non-AGE controls, respectively (Table 2). VE was 91.6% (95% CI: 74.6%, 98.3%) when the control groups were combined. VE against G1P RV hospitalization was 94.5% (95% CI: 65.7%, 99.9%), 95% (95% CI: 70.5%, 99.9%) and 94.6% (95% CI: 68.8%, 99.9%) with RV-negative, non-AGE and combined controls, respectively.
A 3-dose series of RotaTeq had been given to 0% of case-patients versus 10.6% and 12% of RV-negative and non-AGE controls, respectively (P < 0.0001; Table 1). Estimated VE of a full 3-dose series against RV gastroenteritis resulting in hospitalization was 96.8% (95% CI: 82.3%, 100%) and 97.1% (95% CI: 84%, 100%) with RV-negative and non-AGE controls, respectively. VE was 97% (95% CI: 83.5%, 100%) when the control groups were combined (Table 2). VE against G1P RV hospitalization was 94.2% (95% CI: 67.6%, 100%), 93.8% (95% CI: 65.3%, 100%) and 94.3% (95% CI: 68.5%, 100%) with RV-negative, non-AGE and combined controls, respectively.
For sensitivity analyses conducted to determine the impact of excluding 127 controls without vaccination history, VE estimates for both vaccines either increased or decreased slightly, while CIs remained within the same range. VE point estimate ranges with RV-negative, non-AGE and combined controls for each model were as follows: (1) 127 controls unvaccinated: 89.8–91.7% for Rotarix and 96.7–96.8% for RotaTeq; (2) 127 controls fully vaccinated with Rotarix: 90.4–92.5% for Rotarix and 97.9–98.5% for RotaTeq; (3) 127 control fully vaccinated with RotaTeq: 92.8–95.3% for Rotarix and 96.9–97.1% for RotaTeq.
To our knowledge, this is one of a very few, head-to-head comparisons of the field effectiveness of both RV vaccines in one country and the only one conducted in an Asian country. Our analysis demonstrates that both RV vaccines provide greater than 90% protection against severe RV gastroenteritis hospitalization among infants in Taiwan. While the point estimates of effectiveness of the full series of Rotarix and RotaTeq differed by about ~8%, the substantial overlap in the CIs precludes any distinction between the 2 vaccines. In addition, the 14% RV detection rate among children 8–35 months of age in this study is much lower than the ~25–40% RV detection rate seen in studies conducted before RV vaccine introduction in Taiwan.4,6 This lower detection rate likely reflects partial coverage with RV vaccines that have >90% effectiveness against severe RV gastroenteritis.
Although the 3 sentinel surveillance hospitals serve families from a range of socioeconomic backgrounds, including those with low to high incomes, our findings are comparable with vaccine efficacy estimates from clinical trials and VE estimates from studies conducted in other high-income countries. In clinical trials conducted in the Europe and Asia, Rotarix vaccine efficacy against severe RV AGE was 90–96%7,16,17; in the United States and Europe, RotaTeq vaccine efficacy was 94–98%.9,17,18 Rotarix VE has been estimated to be 75–97% in Spain,13,19 while RotaTeq VE has been estimated to be 81–95% in Spain and the United States.10,13,19–21 Of note, the only other published VE studies comparing the simultaneous introduction of both Rotarix and RotaTeq were conducted in Spain.13,19
This study has some limitations. First, not all children who presented with RV-negative AGE or for non-AGE visits were included in the final analysis due to lack of vaccination history confirmation, and not all children enrolled as controls were matched to a case-patient by date of birth within 1 month. Any potential difference between these children and those included in the final analysis may have affected our VE estimates. An analysis of potential differences between excluded versus included children in each control group indicate no major differences in demographic characteristics, except that the relative distribution of hospital location differed between excluded and included non-AGE controls. There were no significant differences in age, sex, prematurity, mother’s education, season of enrollment or number of family members between excluded and included controls (data not shown). Also, given the comparable VE estimates among the 2 control groups and the comparable VE estimates seen in the sensitivity analysis including the excluded controls, the exclusion of these children likely was not a major factor. Second, case-patients differed from controls by season of enrollment. The fact that a greater proportion of case-patients were enrolled during the winter season compared with children enrolled as controls is likely a reflection of the seasonality of RV disease in Taiwan. Also, case-patients and RV-negative AGE controls differed from non-AGE controls by maternal educational background. Mothers of non-AGE controls tended to report a college education or higher more frequently than mothers of case-patients and RV-negative AGE controls. The reasons for this are unclear, but could reflect a better understanding of how to manage AGE at home among mothers with higher educational backgrounds. Third, enrollment was limited to 3 surveillance sites in 3 major cities, so results may not be generalizable to the entire country. Finally, G1P RV accounted for many RV-positive cases. Therefore, we were unable to assess VE against additional strains.
This post-licensure study has demonstrated that both currently licensed RV vaccines provide excellent protection against severe RV gastroenteritis hospitalization among Taiwanese infants, similar to that seen in prelicensure clinical trials and postmarketing evaluations in other high- and middle-income countries. Currently in the private sector in Taiwan, a complete 2-dose course of Rotarix costs approximately US$172 and a full 3-dose course of RotaTeq costs approximately US$200, costs too high to allow some parents to vaccinate their children against RV disease, as likely reflected by the RV vaccination coverage of ~24–28% among our study controls. The addition of RV vaccination into Taiwan’s National Immunization Program has the potential to result in greater RV vaccine coverage through publically funded vaccination. This in turn would lead to the prevention of a substantial number of AGE hospitalizations among children <3 years of age.
Our findings provide compelling evidence of the potential benefits of RV vaccination among infants, and they should help inform policymakers in Taiwan and other similar Asian countries when deciding whether to include RV vaccination into their national immunization programs.
We would like to thank Ms. Ching-Yi Wu (National Health Research Institutes, Taiwan) for assistance in performing RV RT-PCR testing; Ms. Shiau-Mei Tsai (Changhua Christian Hospital, Taiwan), the Chang Gung Memorial Hospital Team (Taoyuan, Taiwan) and the Kaohsiung Chang Gung Memorial Hospital Team (Kaohsiung, Taiwan) for collecting the stool samples and confirming the vaccination history and Dr. Jon Gentsch and Dr. Baoming Jiang for their technical advice on RV specimen testing.
1. Parashar UD, Burton A, Lanata C, et al. Global mortality associated with rotavirus disease among children in 2004. J Infect Dis. 2009;200(suppl 1):S9––S15
2. Parashar UD, Hummelman EG, Bresee JS, et al. Global illness and deaths caused by rotavirus disease in children. Emerg Infect Dis. 2003;9:565–572
3. Tate JE, Burton AH, Boschi-Pinto C, et al. 2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis. Lancet Infect Dis. 2012;12:136–141
4. Chen KT, Chen PY, Tang RB, et al. Sentinel hospital surveillance for rotavirus diarrhea in Taiwan, 2001–2003. J Infect Dis. 2005;192(suppl 1):S44––S48
5. Chen SY, Chang YC, Lee YS, et al. Molecular epidemiology and clinical manifestations of viral gastroenteritis in hospitalized pediatric patients in Northern Taiwan. J Clin Microbiol. 2007;45:2054–2057
6. Wu FT, Liang SY, Tsao KC, et al. Hospital-based surveillance and molecular epidemiology of rotavirus infection in Taiwan, 2005–2007. Vaccine. 2009;27(suppl 5):F50––F54
7. Phua KB, Lim FS, Lau YL, et al. Safety and efficacy of human rotavirus vaccine during the first 2 years of life in Asian infants: randomised, double-blind, controlled study. Vaccine. 2009;27:5936–5941
8. Ruiz-Palacios GM, Pérez-Schael I, Velázquez FR, et al.Human Rotavirus Vaccine Study Group. Safety and efficacy of an attenuated vaccine against severe rotavirus gastroenteritis. N Engl J Med. 2006;354:11–22
9. Vesikari T, Matson DO, Dennehy P, et al.Rotavirus Efficacy and Safety Trial (REST) Study Team. Safety and efficacy of a pentavalent human-bovine (WC3) reassortant rotavirus vaccine. N Engl J Med. 2006;354:23–33
10. Boom JA, Tate JE, Sahni LC, et al. Effectiveness of pentavalent rotavirus vaccine in a large urban population in the United States. Pediatrics. 2010;125:e199–e207
11. Correia JB, Patel MM, Nakagomi O, et al. Effectiveness of monovalent rotavirus vaccine (Rotarix) against severe diarrhea caused by serotypically unrelated G2P strains in Brazil. J Infect Dis. 2010;201:363–369
12. de Palma O, Cruz L, Ramos H, et al. Effectiveness of rotavirus vaccination against childhood diarrhoea in El Salvador: case-control study. BMJ. 2010;340:c2825
13. Martinón-Torres F, Bouzón Alejandro M, Redondo Collazo L, et al.ROTACOST research team. Effectiveness of rotavirus vaccination in Spain. Hum Vaccin. 2011;7:757–761
14. Patel M, Pedreira C, De Oliveira LH, et al. Association between pentavalent rotavirus vaccine and severe rotavirus diarrhea among children in Nicaragua. JAMA. 2009;301:2243–2251
15. Wu TC, Liu HH, Chen YJ, et al. Comparison of clinical features of childhood norovirus and rotavirus gastroenteritis in Taiwan. J Chin Med Assoc. 2008;71:566–570
16. Kawamura N, Tokoeda Y, Oshima M, et al. Efficacy, safety and immunogenicity of RIX4414 in Japanese infants during the first two years of life. Vaccine. 2011;29:6335–6341
17. Vesikari T, Karvonen A, Prymula R, et al. Efficacy of human rotavirus vaccine against rotavirus gastroenteritis during the first 2 years of life in European infants: randomised, double-blind controlled study. Lancet. 2007;370:1757–1763
18. Vesikari T, Karvonen A, Ferrante SA, Ciarlet M. Efficacy of the pentavalent rotavirus vaccine, RotaTeq(R), in Finnish infants up to 3 years of age: the Finnish Extension Study. Eur J Pediatr. 2010;169:1379–1386
19. Castilla J, Beristain X, Martínez-Artola V, et al. Effectiveness of rotavirus vaccines in preventing cases and hospitalizations due to rotavirus gastroenteritis in Navarre, Spain. Vaccine. 2012;30:539–543
20. Boom JA, Tate JE, Sahni LC, et al. Sustained protection from pentavalent rotavirus vaccination during the second year of life at a large, urban United States pediatric hospital. Pediatr Infect Dis J. 2010;29:1133–1135
21. Cortese MM, Leblanc J, White KE, et al. Leveraging state immunization information systems to measure the effectiveness of rotavirus vaccine. Pediatrics. 2011;128:e1474–e1481
gastroenteritis; rotavirus; rotavirus vaccine; vaccine effectiveness
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