Skip Navigation LinksHome > April 2013 - Volume 32 - Issue 4 > A retrospective analysis of nosocomial viral gastrointestina...
Pediatric Infectious Disease Journal:
doi: 10.1097/INF.0b013e318291a62b
Erratum

A retrospective analysis of nosocomial viral gastrointestinal and respiratory tract infections: ERRATUM

Free Access

In the article on page 1233 of volume 31, issue 12 of The Pediatric Infectious Disease Journal, the authorship and text are missing content. Two additional authors belong in the author listing for this article, the authors should be listed as Jan A. Sidler, BMed,* Cedric Haberthür, BA,* Alexis Dumoulin, PhD,† Hans H. Hirsch, MD, MS,†‡ and Ulrich Heininger, MD*. The affiliations for the authors are as follows: *Division of Pediatric Infectious Diseases, University Children’s Hospital, Basel, Switzerland; †Division Infection Diagnostics ('Institute for Medical Microbiology'), Department Biomedicine – Haus Petersplatz, University of Basel, Basel, Switzerland; and ‡Division Infectious Diseases & Hospital Epidemiology, University Hospital Basel, Basel, Switzerland. The Laboratory Assays section within the METHODS, should also contain the text and references 37 and 38 listed below.

Back to Top | Article Outline

Laboratory Assays

Upper respiratory tract specimens were obtained from all patients with new onset of RTI during hospitalization by a standardized approach using sterile flocked swabs for pediatric nasal sampling (Copan FLOQSwabs Ultra Mini Tip 516CS01, Brescia, Italy). These were placed in 2 ml of virus transport medium (Hanks balanced salt [Life technologies, Zug, Switzerland] containing 1% bovine serum albumin and Phenol Red). The specimens were refrigerated at 4°C until total nucleic acids were extracted from 200 μL of specimen using the QIAxtractor automated extraction system and the VX viral nucleic acids extraction kit (Qiagen, Hombrechtikon, Switzerland) according to the manufacturer’s instructions. The elution volume was 200 uL, and 10 uL were used for the multiparameter RespiFinder PCR assay (Pathofinder, Maastricht, The Netherlands).

This assays is based on the multiplex ligation-dependent probe amplification technology which detects and differentiates 14 RNA viruses, 1 DNA virus as well as 4 bacteria in a single assay: Adenovirus, Bordetella pertussis, Chlamydophila pneumoniae, coronavirus 229E, coronavirus NL63, coronavirus OC43, human metapneumovirus, influenza virus A, influenza virus A/H5N1, influenza virus B, Legionella pneumophila, Mycoplasma pneumoniae, parainfluenza viruses 1, 2, 3, and 4, RSV-A, RSV-B and rhinovirus.37,38

Stool specimens were collected from all children with new onset of gastroenteritis during hospitalization and tested for rotavirus and adenovirus antigens using a combined rapid latex agglutination assay (Diarlex Rota-Adeno, Orion Diagnostica, Espoo, Finland).

37. Dumoulin A, Widmer AF, and Hirsch HH. Comprehensive diagnostics for respiratory virus infections after transplantation or after potential exposure to swine flu A/H1N1: what else is out there? Transpl Infect Dis. 2009;11:287–289. Cited Here...

38. Reijan M, Dingemans G, Klaassen CH, et al. RespiFinder: a new multiparameter test to differentially identify fifteen respiratory viruses. J Clin Microbiol. 2008;46:1232–1240. Cited Here...

Back to Top | Article Outline

REFERENCES

1. Sidler JA, Haberthur C, Heininger U. A retrospective analysis of nosocomial viral gastrointestinal and respiratory tract infections. Pediatr Infect Dis J. 2012;31:1233–1238

© 2013 Lippincott Williams & Wilkins, Inc.

Login