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Comparison of Anti-pneumococcal Antibodies in Cord Blood From Australian Indigenous and Gambian Neonates and the Implications for Otitis Media

Balloch, Anne MSc*; Licciardi, Paul V. PhD*; Kemp, Andrew S. PhD*; Leach, Amanda J. PhD; Mulholland, E. Kim MBBS*†‡; Tang, Mimi LK PhD*§¶

Pediatric Infectious Disease Journal:
doi: 10.1097/INF.0000000000000202
Pathogenesis and Host Response
Abstract

Background: Australian indigenous infants experience the highest incidence of chronic suppurative and acute otitis media in the world with many babies developing disease in the early postnatal period. Streptococcus pneumoniae is the major cause of otitis media in this population. Infants are protected against bacterial disease in the first months of life by passive transfer of maternal antibody across the placenta during the late stages of gestation. We hypothesized that reduced passive immunity may contribute to increased disease risk in this population. We compared the concentrations and function of serotype-specific IgG in cord serum from Australian indigenous neonates and Gambian neonates, the latter experiences a similar socioeconomic status to Australian indigenous neonates.

Methods: Serotype-specific IgG, IgG1 and IgG2 were measured using a modified 3rd generation enzyme linked immunosorbent assay based on World Health Organization recommendations. Antibody avidity was measured using a modified sodium thiocyanate elution method.

Results: Australian indigenous neonates had significantly increased levels of serotype-specific IgG compared with Gambian populations for 6 of 12 serotypes (P < 0.02). There was no significant difference in antibody function, as measured by antibody avidity, between the 2 groups.

Conclusions: An increased risk for otitis media in Australian indigenous neonates is not primarily determined by specific antibody titers against pneumococcal bacteria. Further investigation into the possible roles of the innate immune response and Eustachian tube dysfunction in the development of chronic otitis media amongst Australian indigenous infants is warranted.

Author Information

From the *Murdoch Childrens Research Institute, Melbourne; Menzies School of Health Research, Royal Darwin Hospital, Darwin, Australia; London School of Hygiene and Tropical Medicine, University of London, London, United Kingdom; §Royal Children’s Hospital, Melbourne; and Department of Paediatrics, The University of Melbourne, Parkville, Australia.

Accepted for publication October 30, 2013.

This project was supported by the Victorian Government’s Operational Infrastructure Support Program and The Financial Markets Foundation for Children, Sydney, Australia (Project 2008–179). The Foundation had no involvement in study design, data collection, analysis and interpretation, writing of the report or decision to submit the paper for publication. The authors have no other funding or conflicts of interest to disclose.

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Address for correspondence: Anne Balloch, MSc, Murdoch Childrens Research Institute, Melbourne, Australia. E-mail: anne.balloch@mcri.edu.au

© 2014 by Lippincott Williams & Wilkins, Inc.