Institutional members access full text with Ovid®

Early Detection of HIV Infection Among Kenyan Infants Using a Reverse Transcriptase Activity Assay

Sivapalasingam, Sumathi MD*; Ahmed, Aabid MBChB; Mendillo, Megan MPH*; Holzman, Robert MD*; Marshed, Fatuma RN; Mwamzuka, Musa BSc; Khamadi, Samuel PhD; Mwau, Matilu PhD; Laverty, Maura RN*; Valentine, Fred MD*

Pediatric Infectious Disease Journal: July 2012 - Volume 31 - Issue 7 - p 732–735
doi: 10.1097/INF.0b013e318259bc1e
HIV Reports

Two hundred HIV-exposed Kenyan infants were tested for HIV infection at birth and at age 6, 12, 24 and 48 weeks, by DNA polymerase chain reaction (PCR) and Cavidi reverse transcriptase (RT) assays and after age 18 months by HIV antibody test. Eleven (5.5%) infants became HIV infected. In 6 infants, positive RT preceded positive DNA PCR. The use of RT assay may facilitate earlier HIV diagnosis in infants.

From the *Department of Medicine, Division of Infectious Diseases, New York University School of Medicine, New York, New York; Bomu Medical Centre, Mombasa, Kenya; and Kenya Medical Research Institute, Nairobi, Kenya.

Accepted for publication April 2, 2012.

This research was supported in part by a grant from the National Center for Research Resources, National Institutes of Health [1UL1RR029893]; NYU Center for AIDS Research Supplemental grant [3 P30 AI27742-15S2]; and an unrestricted grant from the Gilead Foundation. The authors have no conflict of interest to disclose.

Address for correspondence: Sumathi Sivapalasingam, MD, NYU School of Medicine450 First Avenue, BCD, Room 649, New York, NY 10016. E-mail: sumathi.sivapalasingam@gmail.com.

Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal’s website (www.pidj.com).

© 2012 Lippincott Williams & Wilkins, Inc.