Background: Rotaviruses are the major cause of acute gastroenteritis in young children worldwide, and require careful surveillance, especially in the context of vaccination programs. Prospective surveillance is required to monitor and characterize rotavirus infections, including viral and clinical data, and to detect the emergence of potentially epidemic strains.
Methods: Between 2006 and 2009, stool samples and clinical records were collected from 2044 children with acute diarrhea admitted to the pediatric emergency units of 13 French university hospitals. Rotaviruses were detected in stools, then genotyped by reverse transcription-polymerase chain reaction with regard to their outer capsid proteins VP4 and VP7.
Results: The genotyping of 1947 rotaviruses showed that G1 (61.7%) and G9 (27.4%) strains were predominant and stable, followed by G2 (6.5%), G3 (4.0%), and G4 (2.5%) strains. Most strains were associated with P (92.9%). Overall, 31 uncommon strains and possible zoonotic reassortants were detected including G12 and G8 strains, some being closely related to bovine strains. No difference in clinical presentation and severity was found among genotypes.
Conclusions: The relative stability of rotavirus genotypes currently cocirculating in France may ensure vaccine effectiveness in the short and medium term. However, the likely emergence of G12 and G8 strains should be monitored during ongoing and future vaccination programs, especially as all genotypes can cause severe infections. Special attention should be paid to the emergence of new rotavirus reassortants not included in current rotavirus vaccines.
From the *Laboratory of Virology, National Reference Centre for Enteric Viruses, Dijon, France; and Department of Pediatrics and Laboratory of Virology, †University Hospital of Dijon, University of Bourgogne, Dijon, France; ‡University Hospital of Saint-Etienne, Saint-Etienne, France; §University Hospital of Brest, Brest, France; ¶Saint-Vincent-de-Paul Hospital, Assistance Public-Hôpitaux de Paris, Paris Descartes University, Paris, France; ∥University Hospital of Nantes, Nantes, France; **Robert-Debré Hospital, Assistance Public-Hôpitaux de Paris, Paris, France; ††University Hospital of Montpellier, Montpellier, France; ‡‡Femme-Mère-Enfant Hospital, Hospices Civils de Lyon, Lyon, France; §§Armand-Trousseau Hospital, Assistance Public-Hôpitaux de Paris, Paris, France; ¶¶University Hospital of Limoges, Limoges, France; ∥∥University Hospital of Poitiers, Poitiers, France; ***University Hospital of Lille, Lille, France; and †††University Hospital of Rennes, Rennes, France.
Accepted for publication June 24, 2010.
Supported by Sanofi-Pasteur-MSD (Lyon, France), the European Rotavirus Network (EuroRotaNet), the University Hospital of Dijon, and the National Reference Center for Enteric Viruses.
Address for correspondence: Alexis de Rougemont, MD, MSc, or Pierre Pothier, MD, PhD, Centre National de Référence des virus entériques, Laboratoire de Virologie, Plateau Technique de Biologie, Centre Hospitalier Universitaire de Dijon, 2 rue Angélique Ducoudray, BP 37013, F-21070 Dijon Cedex, France. E-mail: email@example.com or firstname.lastname@example.org.
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