Skip Navigation LinksHome > February 2011 - Volume 30 - Issue 2 > Molecular and Clinical Characterization of Rotavirus From Di...
Pediatric Infectious Disease Journal:
doi: 10.1097/INF.0b013e3181ef034e
Original Studies

Molecular and Clinical Characterization of Rotavirus From Diarrheal Infants Admitted to Pediatric Emergency Units in France

de Rougemont, Alexis MD, MSc*†; Kaplon, Jérôme BSc*; Pillet, Sylvie PharmD, PhD‡; Mory, Olivier MD‡; Gagneur, Arnaud MD, PhD§; Minoui-Tran, Adissa PharmD§; Meritet, Jean-François PharmD¶; Mollat, Claudine PharmD∥; Lorrot, Mathie MD, MSc**; Foulongne, Vincent PharmD, PhD††; Gillet, Yves MD, PhD‡‡; Nguyen-Bourgain, Christelle MD§§; Alain, Sophie MD, PhD¶¶; Agius, Gérard MD, PhD∥∥; Lazrek, Mouna PharmD***; Colimon, Ronald MD, PhD†††; Fontana, Caroline BSc*; Gendrel, Dominique MD, PhD¶; Pothier, Pierre MD, PhD*†; the French Rotavirus Network

Supplemental Author Material
Collapse Box

Abstract

Background: Rotaviruses are the major cause of acute gastroenteritis in young children worldwide, and require careful surveillance, especially in the context of vaccination programs. Prospective surveillance is required to monitor and characterize rotavirus infections, including viral and clinical data, and to detect the emergence of potentially epidemic strains.

Methods: Between 2006 and 2009, stool samples and clinical records were collected from 2044 children with acute diarrhea admitted to the pediatric emergency units of 13 French university hospitals. Rotaviruses were detected in stools, then genotyped by reverse transcription-polymerase chain reaction with regard to their outer capsid proteins VP4 and VP7.

Results: The genotyping of 1947 rotaviruses showed that G1 (61.7%) and G9 (27.4%) strains were predominant and stable, followed by G2 (6.5%), G3 (4.0%), and G4 (2.5%) strains. Most strains were associated with P[8] (92.9%). Overall, 31 uncommon strains and possible zoonotic reassortants were detected including G12 and G8 strains, some being closely related to bovine strains. No difference in clinical presentation and severity was found among genotypes.

Conclusions: The relative stability of rotavirus genotypes currently cocirculating in France may ensure vaccine effectiveness in the short and medium term. However, the likely emergence of G12 and G8 strains should be monitored during ongoing and future vaccination programs, especially as all genotypes can cause severe infections. Special attention should be paid to the emergence of new rotavirus reassortants not included in current rotavirus vaccines.

© 2011 Lippincott Williams & Wilkins, Inc.

Login

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.