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TEST CHARACTERISTICS OF COMMERCIAL INFLUENZA ASSAYS FOR DETECTING PANDEMIC INFLUENZA A (H1N1) IN CHILDREN

Sandora, Thomas J. MD, MPH*; Smole, Sandra C. PhD†; Lee, Grace M. MD, MPH*‡; Chung, Sarita MD§; Williams, Laura BS¶; McAdam, Alexander J. MD, PhD¶

Pediatric Infectious Disease Journal:
doi: 10.1097/INF.0b013e3181be9f9c
Brief Reports
Abstract

We assessed the test characteristics of 2 influenza antigen tests, a rapid immunoassay and a direct fluorescence antibody (DFA) assay, in detecting pandemic influenza A (H1N1) in children up to 18 years of age, using polymerase chain reaction as the standard. The sensitivities of BinaxNOW (59.6%) and direct fluorescence antibody (57.3%) were similar. The specificity of each test was >99%.

Author Information

From the *Division of Infectious Diseases, Departments of Medicine and Laboratory Medicine, Children's Hospital Boston, Boston, MA; †Hinton State Laboratory Institute, Massachusetts Department of Public Health, Jamaica Plain, MA; ‡Department of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care Institute, Boston, MA; §Center for Biopreparedness, Division of Emergency Medicine, Children's Hospital Boston, Boston, MA; and ¶Division of Infectious Diseases Diagnostics, Department of Laboratory Medicine, Children's Hospital Boston, Boston, MA.

Accepted for publication August 28, 2009.

The authors did not receive any source of support.

Address for correspondence: Thomas J. Sandora, MD, MPH, Children's Hospital Boston, 300 Longwood Avenue, Boston, MA 02115. E-mail: thomas.sandora@childrens.harvard.edu.

© 2010 Lippincott Williams & Wilkins, Inc.