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Age dependence of in vitro survival of meningococci in whole blood during childhood

ISON, CATHERINE A. PhD; ANWAR, NATASHA PhD; COLE, MICHELLE J. BSC; POLLARD, ANDREW J. FRCPCH, PhD; MORLEY, SARAH L. MRCP; FIDLER, KATY MBBS; SANDIFORD, COLIN PhD; BANKS, JULIA PhD; KROLL, SIMON J. FRCP; LEVIN, MICHAEL FRCP, PhD

The Pediatric Infectious Disease Journal: October 2003 - Volume 22 - Issue 10 - p 868-874
doi: 10.1097/01.inf.0000091283.10199.dc
Original Studies

Objectives. To determine the association between the ability of a different strains of meningococci to survive in whole blood and the age of the donor.

Methods. A panel of serogroup B and a serogroup C strain of Neisseria meningitidis was tested in an ex vivo whole blood model. Blood from 81 healthy children and 20 adults and from children during convalescence from serogroup B (55 patients) or serogroup C (43 patients) meningococcal infection was assessed.

Results. Age-dependent acquisition of whole blood killing of serogroup B and C bacterial isolates was demonstrated in healthy children with an inverse relationship to the reported incidence of disease. After infection with serogroup B or C meningococci, evidence of whole blood killing of the bacteria was found even in blood from children <2 years of age, the survival of a serogroup B strain, MC58, being reduced compared with that in healthy children (median, 64% compared with 194.5% survival at 90 min). In both affected children and controls, there was a significant correlation between whole blood killing of strain MC58 and of other serogroup B and C meningococci.

Conclusions. The whole blood model measures both humoral and cellular mechanisms responsible for the bactericidal activity of blood. The model was first described 80 years ago, but this is the first description of its age dependency. Acquisition of bactericidal activity was more rapid in children infected and is directed at various strains of meningococci, indicating the presence of a cross-reactive antigen(s).

From the Departments of Infectious Diseases and Microbiology (CAI, NA, MJC, CS, JB) and Paediatrics (NA, MJC, AJP, SLM, CS, JB, SJK, ML), Faculty of Medicine, Imperial College London, and the Department of Paediatrics, Northwick Park Hospital, NHS Trust (KF), London, UK.

Accepted for publication June 19, 2003.

Current address: Department of Paediatrics, University of Oxford, Level 4, John Radcliffe Hospital, Oxford OX3 9DU, UK.

Current address: Infectious Diseases and Microbiology Unit, Institute of Child Health, 30 Guildford Street, London WC1N 1EH.

Reprints not available.

© 2003 Lippincott Williams & Wilkins, Inc.