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Chitin as a Scaffold for Mesenchymal Stem Cells Transfers in the Treatment of Partial Growth Arrest

Li, Li MD; Hui, James Hoi Po FRCS; Goh, James Cho Hong PhD; Chen, Fen MBBS; Lee, Eng Hin MD, FRCS

Journal of Pediatric Orthopaedics: March-April 2004 - Volume 24 - Issue 2 - p 205-210
Growth: Original Article

To investigate the feasibility of using chitin, a natural polymer, as a scaffold to repair large growth plate defects (50% of physis) in immature rabbits, mesenchymal stem cells (MSCs) were harvested from periosteum. The compatibilities of chitin with the MSCs were investigated in vitro using immunohistochemistry and fluorescence confocal microscopy. The results showed high compatibilities of chitin. An experimental model of growth arrest was created by excising the medial half of the proximal growth plate of the tibia in 6-week-old New Zealand White rabbits. The physeal defect after excision of the bony bridge was transplanted either with no interposition (group 1), chitin alone (group 2), or chitin with MSCs (group 3). In groups 2 and 3, both angulatory deformities and length discrepancies of the tibia were corrected. The differences between group 3 and group 1 were greater than those between group 2 and group 1.

Study conducted at the National University of Singapore, Republic of Singapore.

From the Department of Orthopaedic Surgery, National University of Singapore, Singapore.

Supported by the National Medical Research Council of Singapore.

Reprints: James Hui, Department of Orthopaedic Surgery, Faculty of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 119074 (e-mail: doshuij@nus.edu.sg).

© 2004 Lippincott Williams & Wilkins, Inc.