Skip Navigation LinksHome > September/October 2002 - Volume 22 - Issue 5 > Muscle-Based Gene Therapy and Tissue Engineering for Treatme...
Text sizing:
A
A
A
Journal of Pediatric Orthopaedics:
Trauma

Muscle-Based Gene Therapy and Tissue Engineering for Treatment of Growth Plate Injuries

Lee, Chang Woo M.D.*; Martinek, Vladimir M.D.*; Usas, Arvydas M.D.*; Musgrave, Doug M.D.*; Pickvance, E. A. M.D.*; Robbins, Paul Ph.D.†; Moreland, Morey S. M.D.*; Fu, Freddie H. M.D.*; Huard, Johnny Ph.D.*†

Collapse Box

Abstract

Growth plate injuries may lead to a progressive angular deformity or longitudinal growth disturbance. The authors investigated the feasibility of gene therapy and tissue engineering based on autologous muscle-and adenoviral-mediated gene transfer of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2) to treat tibial physeal defects in rabbits. The medial half of the left proximal tibial growth plate was completely excised in 44 6-week-old New Zealand white rabbits. Four experimental groups were created: no treatment (I), autologous muscle interposition (II), autologous muscle interposition injected with adIGF-1 (III), and autologous muscle interposition injected with adBMP-2 (IV). Radiographic and histologic assessments were obtained postoperatively. Significant tibial shortening and a compact osseous bridge were observed in groups I and IV. Growth plates remained open in groups II and III. This experiment demonstrates that IGF-1 had a supportive effect on physeal chondrocytes, while BMP-2 caused increased osteogenic activity in the injured growth plates.

© 2002 Lippincott Williams & Wilkins, Inc.

The Pediatric Orthopaedic Society of North America (POSNA)
is a group of healthcare professionals, primarily pediatric orthopaedic surgeons, dedicated to advancing musculoskeletal care of children and adolescents. JPO is our official member journal. 
Like us on facebook and become a member today!

Login

Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.