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Journal of Pediatric Orthopaedics:
Trauma

Muscle-Based Gene Therapy and Tissue Engineering for Treatment of Growth Plate Injuries

Lee, Chang Woo M.D.*; Martinek, Vladimir M.D.*; Usas, Arvydas M.D.*; Musgrave, Doug M.D.*; Pickvance, E. A. M.D.*; Robbins, Paul Ph.D.†; Moreland, Morey S. M.D.*; Fu, Freddie H. M.D.*; Huard, Johnny Ph.D.*†

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Abstract

Growth plate injuries may lead to a progressive angular deformity or longitudinal growth disturbance. The authors investigated the feasibility of gene therapy and tissue engineering based on autologous muscle-and adenoviral-mediated gene transfer of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2) to treat tibial physeal defects in rabbits. The medial half of the left proximal tibial growth plate was completely excised in 44 6-week-old New Zealand white rabbits. Four experimental groups were created: no treatment (I), autologous muscle interposition (II), autologous muscle interposition injected with adIGF-1 (III), and autologous muscle interposition injected with adBMP-2 (IV). Radiographic and histologic assessments were obtained postoperatively. Significant tibial shortening and a compact osseous bridge were observed in groups I and IV. Growth plates remained open in groups II and III. This experiment demonstrates that IGF-1 had a supportive effect on physeal chondrocytes, while BMP-2 caused increased osteogenic activity in the injured growth plates.

© 2002 Lippincott Williams & Wilkins, Inc.

The Pediatric Orthopaedic Society of North America (POSNA)
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