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Fully implantable, battery-free wireless optoelectronic devices for spinal optogenetics

Samineni, Vijay K.a,b; Yoon, Jangyeolc; Crawford, Kaitlyn E.c; Jeong, Yu Rad; McKenzie, Kajanna Ca,b; Shin, Gunchulc; Xie, Zhaoqiane,f; Sundaram, Saranya S.a,b; Li, Yuhangg; Yang, Min Youngc; Kim, Jeonghyunc; Wu, Die,f; Xue, Yeguange; Feng, Xuef; Huang, Yonggange; Mickle, Aaron D.a,b; Banks, Anthonyc; Ha, Jeong Sookd; Golden, Judith P.a,b; Rogers, John A.c,h; Gereau, Robert W. IVa,b,*

doi: 10.1097/j.pain.0000000000000968
Research Paper
Editor's Choice
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The advent of optogenetic tools has allowed unprecedented insights into the organization of neuronal networks. Although recently developed technologies have enabled implementation of optogenetics for studies of brain function in freely moving, untethered animals, wireless powering and device durability pose challenges in studies of spinal cord circuits where dynamic, multidimensional motions against hard and soft surrounding tissues can lead to device degradation. We demonstrate here a fully implantable optoelectronic device powered by near-field wireless communication technology, with a thin and flexible open architecture that provides excellent mechanical durability, robust sealing against biofluid penetration and fidelity in wireless activation, thereby allowing for long-term optical stimulation of the spinal cord without constraint on the natural behaviors of the animals. The system consists of a double-layer, rectangular-shaped magnetic coil antenna connected to a microscale inorganic light-emitting diode (μ-ILED) on a thin, flexible probe that can be implanted just above the dura of the mouse spinal cord for effective stimulation of light-sensitive proteins expressed in neurons in the dorsal horn. Wireless optogenetic activation of TRPV1-ChR2 afferents with spinal μ-ILEDs causes nocifensive behaviors and robust real-time place aversion with sustained operation in animals over periods of several weeks to months. The relatively low-cost electronics required for control of the systems, together with the biocompatibility and robust operation of these devices will allow broad application of optogenetics in future studies of spinal circuits, as well as various peripheral targets, in awake, freely moving and untethered animals, where existing approaches have limited utility.

Here we demonstrate a fully implantable optoelectronic device powered by near-field wireless communication technology for optogenetic studies in the spinal cord.

aWashington University Pain Center and

bDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, MO, USA, Washington University School of Medicine, St. Louis, MO, USA

cDepartment of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA. Kim is now with the Department of Electronics Convergence Engineering, Kwangwoon University, Seoul, Republic of Korea

dDepartment of Chemical and Biological Engineering, KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea

eDepartments of Civil and Environmental Engineering, Mechanical Engineering, Materials Science and Engineering, Northwestern University, Evanston, IL, USA

fAML, Department of Engineering Mechanics, Center for Mechanics and Materials, Tsien Excellent Education Program, School of Aerospace, Tsinghua University, Beijing, China

gSchool of Aeronautic Science and Engineering, Institute of Solid Mechanics, Beihang University (BUAA), Beijing, China

hDepartments of Materials Science and Engineering, Biomedical Engineering, Chemistry, Mechanical Engineering, Electrical Engineering and Computer Science, and Neurological Surgery, Center for Bio-Integrated Electronics, Simpson Querrey Institute for Nano/biotechnology, Northwestern University, Evanston, IL, USA

Corresponding author. Address: Department of Anesthesiology, Washington University School of Medicine, 660 S Euclid Ave, Campus Box 8054, St. Louis, MO, 63110, USA. E-mail address: gereaur@wustl.edu (R. W. Gereau).

Sponsorships or competing interests that may be relevant to content are disclosed at the end of this article.

Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Web site (www.painjournalonline.com).

Received March 17, 2017

Received in revised form May 16, 2017

Accepted May 24, 2017

© 2017 International Association for the Study of Pain
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