In addition, ANCOVA was carried out, taking age as a covariate to show whether age was a probable factor causing the difference in mean scotopic pupil diameter among the three refractive groups. Pairwise ANCOVA revealed that the difference in mesopic pupil size between all refraction groups (p < 0.001) persisted even after age was taken as a covariate. The results of ANCOVA analysis with age as covariate are shown in Table 5.
Bivariate correlations between mesopic pupil size and spherical equivalent, age, average keratometry, and minimal pachymetry were performed via Pearson correlation analysis. Correlation coefficients and p values are shown in Table 6.
Age revealed the strongest correlation to mesopic pupil size in Pearson correlation analysis. Spherical equivalent had a moderate correlation with mesopic pupil size. Correlation between mesopic pupil size and average keratometry/minimal pachymetry was statistically (p < 0.001) significant, but this correlation is only weak (r = −0.064 for keratometry and r = −0.057 for pachymetry) and clinically insignificant. To analyze whether any of the variables that showed a statistically significant association with mesopic pupil size in univariate analysis contributed independently to the variability of these measurements, multivariate regression analyses were performed. Age, refraction (preoperative spherical equivalent), average keratometry, pachymetry, and ocular side were defined as dependent variables. The multivariate regression model (R2 = 0.179 and p < 0.001) showed that all four variables except ocular side had statistically significant effects on the model. Standardized beta coefficient reveals that average keratometry and pachymetry have only a marginal impact on mesopic pupil size, whereas age (beta coefficient −0.38) has the strongest impact on mesopic pupil size of the factors analyzed. Standardized and unstandardized coefficients of these variables are demonstrated in Table 7.
The scattergram in Fig. 5 reveals the relationship between mesopic pupil size and age controlling for refraction state (R2 linear = 0.171).
Since the beginnings of laser refractive surgery >20 years ago, steady improvements in Excimer technology and preoperative diagnostic tools have helped to optimize the treatment outcomes. The severity of quality of vision complaints with small optical zones (OZ) <5.5 mm, especially at night, led surgeons and manufacturers to increase the ablation zone diameter to >6.00 mm.12
Although several studies4,7,10 concluded that large pupil size does not positively correlate with any of postoperative visual symptoms 12 months after LASIK, the role of mesopic pupil size as a risk factor in predicting night vision complaints is still controversial. In general, optical models, such as point spread function calculations, suggest that pupil size should be an important determinant in selecting appropriate ablation zone size for treatment13,14 However, visual perception is multifactorial, and optical models cannot account fully for adaptive mechanisms. Some of these adaptive properties have been defined, such as the Stiles–Crawford effect (directional sensitivity of the retina), whereas others are less well understood. Bühren et al.15 demonstrated that not mesopic pupil size itself but the ratio between the planned OZ and the pupil size (FC) should be considered before treatment. Although large OZ diameters would be desirable, their usefulness is limited because of higher ablations depths required, which carry the risk for iatrogenic keratectasia.16 However, we and others6,15 think that it is counterintuitive to disregard pupil size as a potential risk factor regarding night vision complaints.
Most refractive surgeons agree that a standardized measurement of pupil size under defined background illumination conditions with good repeatability is of importance to screening patients eligible for refractive surgery.17,18 Most Excimer laser platforms base their experience and normograms on an optical zone of 6.0 or 6.5 mm, which correspond to scotopic pupil aperture of 5.5 to 6.0 mm. In our study, we used the Colvard pupillometer to determine mesopic pupil size, and to the best of our knowledge, we present the largest cohort of pupil size measurements with Colvard pupillometer (n = 13,959). Originally, the Colvard device was reported to measure scotopic pupil size,19 but recent literature shows best comparison with Procyon low mesopic pupil size measurement: Bootsma et al.20 showed a stronger correlation of Colvard pupillometry with Procyon measurements taken under mesopic-low light conditions than with Procyon measurements taken under scotopic conditions. Measurements of pupil size taken with the Colvard pupillometer are monocular, and the accuracy may therefore be affected by bilaterality because it is a handheld device.21 On the other hand, Kohnen et al.18 did not show that bilateral pupil size measurement with the Colvard device has a negative measuring bias and results are comparable with a digital (Procyon) device, but the authors summarize that digital pupillometers currently provide the most accurate measurement of the scotopic pupil. For this reason, we analyzed the right and left eye separately and found no significant difference in mean mesopic pupil size between right and left eye (p = 0.197; Student's t-test). In addition, no significant difference in mean mesopic pupil size between male and female subjects was found (p = 0.336; Student's t-test).
The overall mean mesopic pupil size in this large population of refractive surgery candidates was 6.45 ± 0.82 mm (mean age: 36.07 years; n = 13,959), and it is therefore in the middle of the range of the previous reports on scotopic/mesopic pupil size measurement with the Colvard pupillometer.
Kohnen et al. reported a mean Colvard pupil size of 5.78 ± 0.98 mm (1 min dark adaptation and 0.28 lux background illuminance) in a study of 100 eyes of subjects with a mean age of 38.8 years18 and of 5.68 ± 1.07 mm (2 min dark adaptation and background illuminance 18.33 lux) in another study of 100 eyes of subjects with a mean age of 36.8 years.22 In our opinion and according to literature,3,23 these mean values are smaller than expected in the reported age-groups. Bootsma et al. measured a mesopic (Colvard) pupil size of 5.86 ± 0.81 (background illuminance 0.15 lux) in an older population (n = 121) with a mean age of 48.8 years.20 Schmitz et al. reported a mean scotopic pupil size of 6.61 mm in a cohort of 56 subjects with a mean age of 23 years (2 min dark adaptation and background illuminance “complete darkness”),24 which is comparable with our mean mesopic pupil size of 6.66 mm in the <39 years subgroup. For all these pupil size measurements, the mean age of the study population must be taken into consideration. In addition, Bradley et al. reported that a steep and variable learning curve with the Colvard pupillometer is another source for potential bias.25 However, because of the large number of measurements performed in our study, this learning curve should not have a significant impact on the outcome. Schallenberg et al. found no significant change of pupil diameter measured with Colvard pupillometer after 2 min or more of dark adaptation.26
Recently, Cakmak et al. analyzed ocular factors that may affect mesopic pupil size.11 Pupil size measurements were performed in their study with a COAS Ocular Wavefront analyzer in mesopic conditions (0.6 lux). Although this is not a standard method to determine mesopic pupil size, previous studies reported that pupil size measurements with wavefront aberrometers were comparable with standard Colvard and Procyon pupillometers.20,24,27 Cakmak et al. reviewed records of 412 refractive surgery candidates and calculated mean mesopic pupil size (COAS Ocular Wavefront analyzer) to 6.18 mm (mean age: 31.85 years). Cakmak et al. showed that age and magnitude of both spherical and cylindrical refractive error are determinative factors on mesopic pupil size. Pupil size was larger in myopic patients than in hyperopic patients. As the magnitude of spherical refractive error increases, pupil size decreases. It was also confirmed that age was an important factor for mesopic pupil size with a smaller pupil size as the age advances.11
In our large-scale study, mean mesopic pupil size (Colvard pupillometer) was 5.96 ± 0.8 mm in hyperopia, 6.36 ± 0.83 mm in astigmatism, and 6.51 ± 0.8 mm in myopia. The differences in mesopic pupil size between all the refractive groups (myopic astigmatism, hyperopic astigmatism, and high astigmatism) were statistically significant (p < 0.001), and persisted even after age was taken as a covariate (ANCOVA). From the clinical point of view, it must be mentioned that although statistically significant, the difference between myopia and astigmatism regarding mesopic pupil size was only marginal. Correlation analysis showed that not only age (r = −0.405 p < 0.001) and refractive state but also average keratometry and pachymetry (thinnest point) had a statistically significant effect on mesopic pupil size, but this correlation must be considered clinically insignificant. We could show that the sum of all the factors (age, refractive state, keratometry, and pachymetry) analyzed can only predict the expected pupil size in <20% cases because R2 value was considerably low (0.179). On the other hand, >80% of the determinative factors and their impact on mesopic pupil size are not explained by our analysis. Last, but not least, pupil size is influenced by intra-individually variable factors such as alertness, diet, drug use, activity, sleep, and lifestyle factors.28 These effects appear to be limited because Robl et al. showed that using a standardized dark-adaptation protocol, refractive surgery candidates did not show a significant variation in scotopic pupil diameter for two testing sessions.29
To our knowledge, we present the largest study including 13,959 randomly selected eyes of refractive surgery candidates and analyzing the effect of ocular and general parameters on mesopic pupil size.
In summary, the present study has revealed and confirmed that age is the strongest determinative factor on mesopic pupil size in refractive surgery candidates. In addition, magnitude of refractive error and astigmatism also had a statistical significant impact on mesopic pupil size. Although statistically significant, the impact of average keratometry and minimal pachymetry on mesopic pupil size is only marginal and should be further analyzed in future studies.
However, the smaller the impact of individual factors on mesopic pupil size, the more important is the standardization of pupil size measurement with regard to dark adaptation and background illuminance. Prospective studies are warranted.
Stephan J. Linke
Department of Ophthalmology
The authors thank the staff and patients of Care Vision for their support in establishing the anonymized refractive data collection (Hamburg Refractive Data Base). The authors are particularly grateful to Vasyl Druchkiv for supporting and supervising the database and expert statistical analysis. The authors have no financial or proprietary interest in this study. No financial support was received.
No grant support or financial funding was received. All authors do not have, direct or indirect, commercial or proprietary, interest in any device, equipment, instrument, drug, or company mentioned in the article, or in any device competing against any device, equipment, instrument, drug, or company mentioned in the article.
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mesopic pupil size; Colvard pupillometer; refractive state; age; pachymetry© 2012 American Academy of Optometry