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Coinfection with Acanthamoeba and Pseudomonas in Contact LensAssociated Keratitis

Sharma, Reetika*; Jhanji, Vishal; Satpathy, Gita; Sharma, Namrata; Khokhar, Sudarshan; Agarwal, Tushar

doi: 10.1097/OPX.0b013e31827f15b4

Purpose: To report coinfection with Acanthamoeba and Pseudomonas aeruginosa in a case with contact lens–associated keratitis.

Case Report: A 20-year-old woman presented to the emergency department of our hospital with a 4-day history of progressively increasing pain, redness, photophobia, mucopurulent discharge, and diminution of vision in her right eye. She was being treated for contact lens–related Pseudomonas keratitis in another hospital before presentation. Gram stain of corneal scrapings revealed gram-negative bacilli. Both Gram stain and 10% KOH wet mount showed the presence of Acanthamoeba cysts. Microbiological cultures obtained from contact lenses and contact lens storage case showed the presence of Pseudomonas aeruginosa and Acanthamoeba. Topical therapy was started in the form of hourly gentamycin 1.3%, cefazolin 5%, chlorhexidine 0.02%, propamidine 0.1%, polymyxin B 30,000 IU eye drops, and neosporin (neomycin, bacitracin, polymyxin) eye ointment four times a day. Symptomatic improvement was observed within 48 hours, along with a decrease in the density of infiltrates and a reduction in the anterior chamber reaction. Repeat corneal scrapings on day 10 showed Acanthamoeba but no bacilli. Progressive resolution of the infiltrate was noted during the next few days. Epithelialization was complete by day 24, following which the amoebicidal therapy was tapered during the next 4 weeks. Complete resolution of keratitis was achieved after 7 weeks of treatment.

Conclusions: Both P. aeruginosa and Acanthamoeba are potentially devastating causes of microbial keratitis. Our case highlights the importance of considering the possibility of a concurrent infection in cases with contact lens–related keratitis.



Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India (RS, GS, NS, SK, TA); Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong (VJ); and Centre for Eye Research Australia, University of Melbourne, Australia (VJ).

Tushar Agarwal Dr. Rajendra Prasad Centre for Ophthalmic Sciences Room S4 All India Institute of Medical Sciences Ansari nagar New Delhi 110029 India e-mail:

© 2013 American Academy of Optometry