Optometry & Vision Science

Skip Navigation LinksHome > January 2012 - Volume 89 - Issue 1 > Effect of Phospholipid Deposits on Adhesion of Bacteria to C...
Optometry & Vision Science:
doi: 10.1097/OPX.0b013e318238284c
Original Article

Effect of Phospholipid Deposits on Adhesion of Bacteria to Contact Lenses

Babaei Omali, Negar*; Proschogo, Nicholas†; Zhu, Hua†; Zhao, Zhenjun†; Diec, Jennie*; Borazjani, Roya†; Willcox, Mark D. P.†

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Abstract

Purpose. Protein and lipid deposits on contact lenses may contribute to clinical complications. This study examined the effect of phospholipids on the adhesion of bacteria to contact lenses.

Methods. Worn balafilcon A (n = 11) and senofilcon A (n = 11) were collected after daily wear and phospholipids were extracted in chloroform:methanol. The amount of phospholipid was measured by electrospray ionization mass spectrometry. Unworn lenses soaked in phospholipids were exposed to Pseudomonas aeruginosa and Staphylococcus aureus. After 18 h incubation, the numbers of P. aeruginosa or S. aureus that adhered to the lenses were measured. Phospholipid was tested for possible effects on bacterial growth.

Results. A broad range of sphingomyelins (SM) and phosphatidylcholines (PC) were detected from both types of worn lenses. SM (16:0) (m/z 703) and PC (34:2) (m/z 758) were the major phospholipids detected in the lens extracts. Phospholipids did not alter the adhesion of any strain of P. aeruginosa or S. aureus (p > 0.05). Phospholipids (0.1 mg/mL) showed no effect on the growth of P. aeruginosa 6294 or S. aureus 031.

Conclusions. Phospholipids adsorb/absorb to contact lenses during wear, however, the major types of phospholipids adsorbed to lenses do not alter bacterial adhesion or growth.

© 2012 American Academy of Optometry

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