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Demonstration of the retention of 64Cu-ATSM in cardiac myocytes using a novel incubation chamber for screening hypoxia-dependent radiotracers

Handley, Maxwell G.; Medina, Rodolfo A.; Paul, Rowena L.; Blower, Philip J.; Southworth, Richard

Nuclear Medicine Communications:
doi: 10.1097/MNM.0b013e328363f25e
Original Articles
Abstract

Objective: We have designed a low-cost, reusable incubation system that allows cells to be cultured in either plated or suspension culture under complete atmospheric control for radiotracer characterization. We demonstrate its utility here in the first quantification of the hypoxia-dependent accumulation of 64Cu-diacetyl bis(N4-methylthiosemicarbazone) (64Cu-ATSM) in adult rat ventricular myocytes (ARVMs).

Materials and methods: ARVMs were allowed to adhere overnight in 9 cm culture plates (2×105 cells/dish) or were used in suspension culture, placed inside the chamber and equilibrated with either oxic (95 or 21% O2/5% CO2) or anoxic gas (95% N2/5% CO2). 64Cu-ATSM of 100 kBq was administered, and the cells were incubated for 30 or 60 min. Cells were then harvested, counted and fractionated to determine intracellular 64Cu biodistribution.

Results: After 1 h, the average cellular 64Cu retention in plated ARVMs under oxygenated conditions was 23.9±2.5 mBq/cell (95% O2), increasing to 27.3±5.1 under 21% O2 (P<0.05) and to 36.1±3.1 under 0% O2 (P<0.05). When ARVMs were cultured in suspension, normoxic–hypoxic contrast was less marked but still significant [63.2±14.1 vs. 53.4±10.9% mBq/cell after 30 min (P<0.05)]. Sixty percent of tracer accumulated in the cytosol, and, although total cellular retention increased during hypoxia, there was no enrichment in any particular cellular compartment.

Conclusion: This apparatus allows the conduction of radiotracer uptake studies in cells under complete atmospheric control, as evidenced by our first demonstration of the hypoxia-dependent uptake of 64Cu-ATSM in ventricular myocytes. It is ideally suited for screening, validating and characterizing novel hypoxia-selective radiotracers.

Author Information

Department of Imaging Chemistry and Biology, Division of Imaging Sciences and Biomedical Engineering, King’s College London, BHF Centre of Research Excellence, King’s Health Partners, St Thomas’ Hospital, London, UK

Correspondence to Richard Southworth, PhD, Department of Imaging Chemistry and Biology, Division of Imaging Sciences and Biomedical Engineering, King’s College London, BHF Centre of Research Excellence, King’s Health Partners, St Thomas’ Hospital, Lambeth Palace Rd, London SE1 7EH, UK Tel: +44 207 188 5435; fax: +44 207 188 8449; e-mail: richard.southworth@kcl.ac.uk

Received February 5, 2013

Accepted June 6, 2013

© 2013 Wolters Kluwer Health | Lippincott Williams & Wilkins