High intensity exercise induced oxidative stress and skeletal muscle damage in post-pubertal boys and girls: A comparative study.Pal Sangita; Chaki, Biswajit; Chattopadhyay, Sreya; Bandyopadhyay, AmitJournal of Strength & Conditioning Research: Post Acceptance: July 31, 2017 doi: 10.1519/JSC.0000000000002167 Original Research: PDF Only Abstract The purpose of this study was to examine the gender variation in high intensity exercise induced oxidative stress and muscle damage among 44 sedentary post-pubertal boys and girls through estimation of post exercise release pattern of muscle damage markers like creatine kinase (CK), lactate dehydrogenase (LDH), alanine aminio transferase (ALT), Aspartate Aminotranferase (AST) and oxidative stress markers like extent of Lipid Peroxidation (TBARS) and Catalase (CAT) activity. Muscle damage markers like CK, LDH, ALT and AST were measured before, immediately after, 24 and 48 hours after high intensity incremental treadmill running. Oxidative stress markers like TBARS and Catalase activity were estimated before and immediately after the exercise. Lipid peroxidation and serum catalase activity increased significantly in both groups following exercise (p < 0.001) with post exercise values and percentage increase significantly higher in post-pubertal boys as compared to girls (p<0.001). CK and LDH activity also increased significantly above pre- exercise level at 24 and 48 hours after exercise in both the genders (p < 0.001) with values significantly higher for boys than the girls (p<0.001). Although ALT and AST increased significantly in both the groups after exercise, the pattern of post exercise release of these markers were found to be similar in both the groups. Accordingly, it has been concluded from the present investigation that high intensity exercise induces significant oxidative stress and increase indices of skeletal muscle damage in both post-pubertal girls and boys. However, post-pubertal girls are relatively better protected from oxidative stress and muscle damage as compared to the boys of similar age and physical activity level. It is further evident that gender difference may not be apparent for all the biomarkers of muscle damage in this age group. Copyright (C) 2017 by the National Strength & Conditioning Association.