Abstract: Rodríguez-Jiménez, S, Benitez, A, González, MÁG, Feliu, GM, and Maffiuletti, NA. The influence of preset frequency, loading condition, and exercise type on the mechanical behavior of a novel vibratory bar. J Strength Cond Res 28(4): 982–989, 2014—This study aimed to analyze the influence of different vibration frequencies, loading conditions, and exercise types on the mechanical behavior of a novel vibratory bar (VB). Fourteen healthy men were asked to hold the VB during lying row (pulling) and bench press (pushing) static exercise as steadily as possible for 10 seconds with loads of 20, 50, and 80% of the maximum sustained load (MSL) and at preset vibration frequencies (fin) of 20, 35, and 50 Hz. Root mean square vibration acceleration (aRMS), peak-to-peak displacement (D), and frequency (fout) were gained from a 3-dimensional accelerometer fixed to the VB. Increasing vibration frequency (from 20 to 50 Hz) resulted in a progressive and sizeable increase in VB aRMS and fout (both p ≤ 0.001) with smaller variations of D (≤5.9%, p ≤ 0.001). Adding weight to the VB (progressive overload from 20 to 80% MSL) did not affect D and minimally altered aRMS (<4.2%, p = 0.014) and fout (<1.7%, p = 0.002). Altering the type of exercise (pulling vs. pushing) did not affect VB aRMS, D, and fout. In conclusion, this study establishes the validity of a novel VB and legitimates its use for effective and safe upper-body static exercise with a wide range of vibration frequencies and loading conditions in the context of physical training or rehabilitation.