Skip Navigation LinksHome > October 2014 - Volume 75 - Issue 4 > Effects of an Immunomodulatory Therapy and Chondroitinase Af...
doi: 10.1227/NEU.0000000000000447

Effects of an Immunomodulatory Therapy and Chondroitinase After Spinal Cord Hemisection Injury

Grosso, Matthew J. MD*,‡,§; Matheus, Virgilio MD*,¶; Clark, Megan MS; van Rooijen, Nico PhD; Iannotti, Christopher A. MD, PhD#; Steinmetz, Michael P. MD**,‡‡

Collapse Box


BACKGROUND: Individually, immunomodulatory therapy and chondroitinases have demonstrated neuroprotective and potential neuroregenerative effects following spinal cord injury.

OBJECTIVE: To investigate the therapeutic potential of combined immunomodulatory and chondroitin sulfate-glycosaminoglycan degradation therapy in spinal cord injury.

METHODS: A combined immunomodulatory treatment using (1) liposome-encapsulated clodronate (selectively depletes peripheral macrophages), and (2) rolipram (a selective type 4 phosphodiesterase inhibitor), along with the chondroitin sulfate proteoglycan-glycosaminoglycan-degrading enzyme, chondroitinase ABC (ChABC), was assessed for its potential to promote axonal regrowth and improve locomotor recovery following midthoracic spinal cord hemisection injury in adult rats.

RESULTS: We demonstrate that combined treatment with liposomal clodronate, rolipram, and ChABC attenuates macrophage accumulation at the site of injury, reduces axonal die-back of injured dorsal column axons, and produces the greatest improvement in locomotor recovery at 6 weeks postinjury compared with controls and noncombined therapy. Anterograde and retrograde tracing revealed that delivery of clodronate, rolipram, and ChABC did not promote substantial axonal regeneration through the site of injury, although the treatment did limit the extent of axonal die-back. Histological assessments revealed that combined treatment with clodronate/rolipram and/or ChABC resulted in a significant reduction in lesion size and cystic cavitation in comparison with injured controls. Combined clodronate, rolipram, and ChABC treatment reduced the accumulation of macrophages within the injured spinal cord 7 weeks after injury.

CONCLUSION: The present data suggest that delivery of an immunomodulatory therapy consisting of clodronate and rolipram, in combination with ChABC, reduces axonal injury and enhances neuroprotection, plasticity, and hindlimb functional recovery after hemisection spinal cord injury in adult rats.

ABBREVIATIONS: ChABC, chondroitinase ABC

CS-GAG, chondroitin sulfate glycosaminoglycan

CSPG, chondroitin sulfate proteoglycan

DMSO, dimethyl sulfoxide

EC, eriochrome cyanine

i.p., intraperitoneal

NIH, National Institutes of Health

PBS, phosphate-buffered saline

PFA, paraformaldehyde

SEM, standard error of the mean

Copyright © by the Congress of Neurological Surgeons


Article Tools


Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.