OBJECTIVE: To evaluate the reclosure-promoting capacity of a neural stem cell line (F3) and a human bone marrow stem cell line (B10) injected into the amniotic cavity of spinal open neural tube defects (ONTDs) of chick embryos of Hamburger and Hamilton stage 18 or 19.
METHODS: Fifteen chick embryos that survived the procedure were obtained for each of 4 groups: untreated control, F3-, B10-, and HFF-1 (human foreskin fibroblast)–treated groups. Embryos in the control group underwent ONTD surgery but no cell injection.
RESULTS: Compared with the untreated control and HFF-1 groups, the B10 group showed enhanced reclosure at 3, 5, and 7 days after injection, whereas the F3 group did not. B10 cells were not incorporated into reclosed neural tubes but simply covered ONTDs during the process of reclosure. F3 cells did not cover ONTDs. The cell survival of F3 cells exposed to the chick amniotic fluid in vitro for 48 hours was significantly lower than that of B10 cells.
CONCLUSION: The results confirmed that B10 cells enhance reclosure of ONTDs by covering and protecting neural tissues, not by direct cell incorporation. The lack of reclosure capacity in the F3 group may be related to the poor survival of F3 cells in the amniotic fluid.
Department of Neurosurgery, Seoul National University College of Medicine, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea (Lee) (Phi) (Ki Kim) (Cho)
Korea Institute for Regenerative Medicine, Inchon, Republic of Korea, Division of Neurology, Department of Medicine, UBC Hospital, University of British Columbia, British Columbia, Vancouver, Canada (U. Kim)
Medical Research Institute, Chung-Ang University College of Medicine, Seoul, Republic of Korea (Wang)
Reprint requests: Kyu-Chang Wang, MD, PhD, Department of Neurosurgery, Seoul National University College of Medicine, 101 Daehangno, Jongno-gu, Seoul 110–744, Republic of Korea. E-mail: firstname.lastname@example.org
Received, March 24, 2009.
Accepted, January 15, 2010.