Skip Navigation LinksHome > July 2008 - Volume 63 - Issue 1 > ACCURACY OF MOTOR AXON REGENERATION ACROSS AUTOGRAFT, SINGLE...
doi: 10.1227/01.NEU.0000319521.28683.75
Experimental Studies


de Ruiter, Godard C. M.D.; Spinner, Robert J. M.D.; Malessy, Martijn J.A. M.D., Ph.D.; Moore, Michael J. Ph.D.; Sorenson, Eric J. M.D.; Currier, Bradford L. M.D.; Yaszemski, Michael J. M.D., Ph.D.; Windebank, Anthony J. M.D.

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OBJECTIVE: The accuracy of motor axon regeneration becomes an important issue in the development of a nerve tube for motor nerve repair. Dispersion of regeneration across the nerve tube may lead to misdirection and polyinnervation. In this study, we present a series of methods to investigate the accuracy of regeneration, which we used to compare regeneration across autografts and single-lumen poly(lactic-co-glycolic acid) (PLGA) nerve tubes. We also present the concept of the multichannel nerve tube that may limit dispersion by separately guiding groups of regenerating axons.

METHODS: The simultaneous tracing of the tibial and peroneal nerves with fast blue and diamidino yellow was performed 8 weeks after the repair of a 1-cm nerve gap in the rat sciatic nerve to determine the percentage of double-projecting motoneurons. Sequential tracing of the peroneal nerve with diamidino yellow 1 week before repair and fast blue 8 weeks after repair was performed to determine the percentage of correctly directed peroneal motoneurons.

RESULTS: In the cases in which there was successful regeneration across single-lumen nerve tubes, more motoneurons had double projections to both the tibial and peroneal nerve branches after single-lumen nerve tube repair (21.4%) than after autograft repair (5.9%). After multichannel nerve tube repair, this percentage was slightly reduced (16.9%), although not significantly. The direction of regeneration was nonspecific after all types of repair.

CONCLUSION: Retrograde tracing techniques provide new insights into the process of regeneration across nerve tubes. The methods and data presented in this study can be used as a basis for the development of a nerve tube for motor nerve repair.

Copyright © by the Congress of Neurological Surgeons


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