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Induction of NG108‐15 cells differrentiation by human bone marrow stromal cells

Ho, Siu-Hong1; So, Lac-Hue1; Lai, Kwok-On1; Ip, Nancy Y.1; Leung, Mun-Fai2,3

Neurochemistry

THE survival and differentiation of neuronal cells is dependent on factors such as neurotrophins, cytokines and components of extracellular matrix. Bone marrow stromal cells have been shown to support the growth and differentiation of neuroblastoma cells. In an attempt to study the effects of bone marrow stromal cells on neuronal differentiation, we have co-cultured neuroblastoma × glioma hybrid NG108-15 cells with human bone marrow stromal cells. After co-culturing, clones exhibiting morphological differentiated phenotype and high level of neurofilament expression were isolated. Interestingly, these clones maintain their ability to proliferate in contrast to differentiated NG108-15 cells induced by dibutyryladenosine 3′,5′-cyclic monophosphate. These results suggested that bone marrow stromal cells can induce partial differentiation of NG108-15 cells.

1Department of Biology and Biotechnology Research Institute, The Hong Kong University of Science and Technology

2Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin N.T., Hong Kong, China

3Corresponding Author: Mun-Fai Leung

ACKNOWLEDGEMENTS: These studies were supported by the ITDC Industrial Support Fund AF/142/96.

Received 27 January 1998; accepted 19 February 1998

© Lippincott-Raven Publishers.