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The protective effect of autophagy on ischemia/reperfusion-induced hearing loss: implications for sudden hearing loss

Yang, Haidia,c,d,*; Pang, Jiaqia,c,*; Xiong, Haoa,c,d; Sun, Yingfenga,c; Lai, Lana; Chen, Suijuna,c,d; Ye, Yongyia,c; Yang, Zhengfeib; Zheng, Yiqinga,c,d

doi: 10.1097/WNR.0000000000000897
Clinical Neuroscience

The present study aimed to determine the effects of ischemia/reperfusion (IR) injury for the carotid system on hearing, particularly, the role of autophagy in this process. Sixty-three Sprague-Dawley rats were divided randomly into three groups: sham surgery animals (S), temporary carotid artery occlusion (ischemia) for 30 min (I30), and temporary carotid artery occlusion for 60 min (I60). Auditory brainstem response measurements were performed on mice. After 72 h of reperfusion, the microcirculation was measured in mice after ischemia injury. Immunofluorescence was used to examine the expression of caspase-3 and light chain 3B in the cochlear sections. Temporary carotid artery occlusion lasting for 30 (I30) or 60 min (I60) caused significant hearing loss in the ischemia phase. Following a recovery during the postreperfusion phase, the temporal threshold shift occurred in the I30 group, whereas a permanent threshold shift occurred in the I60 group. Moreover, both microcirculation and autophagy affected hearing 24 h after reperfusion, whereas at 72 h, autophagy works as an intrinsic cellular process that protects against death from the IR effect. These results suggest that the sooner the reperfusion, the better the hearing recovery. In conclusion, autophagy promotes cell survival in the cochlea; however, excessive IR damage counteracts the beneficial potential of autophagy protection and leads to a permanent threshold shift.

Departments of aOtolaryngology

bEmergency, Sun Yat-sen Memorial Hospital

cInstitute of Hearing and Speech-Language Science

dDepartment of Hearing and Speech Science, Xinhua College, Sun Yat-Sen University, Guangzhou, China

* Haidi Yang and Jiaqi Pang contributed equally to the writing of this article.

Correspondence to Yiqing Zheng, MD, Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-Sen University, 107 West Yan Jiang Road, Guangzhou 510120, China Tel: +86 20 81332566; fax: +86 20 81332115; e-mail: yiqingzheng@hotmail.com Correspondence to Zhengfei Yang, MD, Department of Emergency, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 West Yan Jiang Road, Guangzhou 510120, China Tel: +86 20 62287686; fax: +86 20 62287686; e-mail: yangzhengfei@vip.163.com

Received June 1, 2017

Accepted July 17, 2017

© 2017 Wolters Kluwer Health | Lippincott Williams & Wilkins