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TLS-GFP cannot rescue mRNP formation near spines and spine phenotype in TLS-KO

Fujii, Ritsukoa; Grossenbacher-Zinchuk, Olgab; Jamari, Ildasolhaa; Wang, Yua; Zinchuk, Vadimc; Takumi, Torud

doi: 10.1097/WNR.0b013e32831bedb0
Developmental Neuroscience

RNA-binding protein TLS transports Nd1-L mRNA, which encodes an actin-stabilizing protein, to the neuronal dendrites. TLS-null mouse (TLS-KO) hippocampal neurons display abnormal spine morphology, and thus could be attributed to actin destabilization by the improper supply of Nd1-L mRNA to the dendrites. In this study, we showed that the exogenous expression of TLS in TLS-KO neurons did not rescue the abnormal spine phenotypes. The degree of colocalization between exogenous TLS and Nd1-L mRNA was significantly decreased in both the neuronal dendrites and the spines of TLS-KO neurons. Our results indicate that formation of TLS–Nd1-L mRNA complex clusters, presumable mRNA pools for the local protein synthesis in the spines, was impaired in TLS-deficient neurons.

aWaseda-Olympus Bioscience Research Institute, Biopolis, Singapore

bInstitute of Anatomy, University of Berne, Berne, Switzerland

cDepartment of Anatomy and Cell Biology, Kochi University, Nankoku, Kochi

dOsaka Bioscience Institute, Osaka, Japan

Correspondence to Ritsuko Fujii, PhD, Waseda-Olympus Bioscience Research Institute, 11 Biopolis Way, Helios #05-01/02, Singapore 138667

Tel: +65 6478 9721; fax: +65 6478 9416; e-mail: fujiir@waseda.jp

Received 13 September 2008 accepted 3 October 2008

© 2009 Lippincott Williams & Wilkins, Inc.