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NRG induces membrane targeting of Gαz in muscle: implication in myogenesis

Lok, Ka-Chun; Fu, Amy K. Y.; Ip, Fanny C. F.; Wong, Yung H.; Ip, Nancy Y.

doi: 10.1097/WNR.0b013e3282e9a5b7
Developmental Neuroscience

The neuregulins (NRGs) constitute a family of trophic factors that are known to play critical roles during neural development. We recently reported that Gβ subunit regulates NRG-mediated signaling and gene transcription in cultured C2C12 myotubes. In this study, we demonstrated that NRG treatment of C2C12 myotubes stimulates a rapid translocation of Gαz protein to the plasma membranes. In addition, Gαz protein is localized to the postsynaptic regions at adult neuromuscular junctions and is prominently expressed in rat skeletal muscle during early postnatal stages. Interestingly, we found that expression of the constitutively activated Gαz in C2C12 myoblasts attenuates myogenic differentiation. Taken together, our observations reveal an unanticipated role of Gαz in mediating the actions of NRG during neural development.

Department of Biochemistry, Biotechnology Research Institute and Molecular Neuroscience Center, Hong Kong University of Science and Technology, Hong Kong, China

Correspondence to Professor Nancy Y. Ip, Department of Biochemistry, Biotechnology Research Institute and Molecular Neuroscience Center, Hong Kong University of Science and Technology, Hong Kong, China

Tel: +852 2358 7304; fax: +852 2358 2765; e-mail: boip@ust.hk

Received 16 May 2007; accepted 30 May 2007

© 2007 Lippincott Williams & Wilkins, Inc.