Caloric restriction mimicked by administration of 2-deoxy-D-glucose (2DG) has been shown to protect cerebral neurons against ischemia and excitotoxicity. This study examined the protective effects of pretreatment with 2DG on retinal neurons in N-methyl-D-aspartate (NMDA) excitotoxicity in rats. There was a significantly reduced number of TUNEL-labeled cells in the retinal ganglion cell layer 18 h after intravitreal injection of NMDA with 2DG pretreatment. At 7 days after NMDA, 2DG pretreatment significantly preserved neurons in the retinal ganglion cell layer and reduced immunoreactivity of glial fibrillary acidic proteins in retinas. Our findings demonstrate that caloric restriction mimicked by 2DG protects retinas from NMDA excitotoxicity.
1Departments of Ophthalmology, Jules Stein Eye Institute
5Neuroscience, University of California Los Angeles School of Medicine, Room B146, 100 Stein Plaza, Los Angeles, CA 90095-7000, USA
2Department of Ophthalmology, Mackay Memorial Hospital, Taipei, Taiwan
3Mackay Junior College of Nursing, Taipei, Taiwan,ROC
4Department of Ophthalmology, Oita Medical University, Oita, Japan
CACorresponding Author: email@example.com
Received 14 July 2003; accepted 3 October 2003