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2-Deoxy-D-glucose protects retinal ganglion cells against excitotoxicity

Lan, Yu-Wen1 2 3; Ishii, Yoko4 5; Palmer, Kimberly E.5; Bristow, Karrah Q.5; Caprioli, Joseph1; Kwong, Jacky M. K.1 CA

Vision, Retina

Caloric restriction mimicked by administration of 2-deoxy-D-glucose (2DG) has been shown to protect cerebral neurons against ischemia and excitotoxicity. This study examined the protective effects of pretreatment with 2DG on retinal neurons in N-methyl-D-aspartate (NMDA) excitotoxicity in rats. There was a significantly reduced number of TUNEL-labeled cells in the retinal ganglion cell layer 18 h after intravitreal injection of NMDA with 2DG pretreatment. At 7 days after NMDA, 2DG pretreatment significantly preserved neurons in the retinal ganglion cell layer and reduced immunoreactivity of glial fibrillary acidic proteins in retinas. Our findings demonstrate that caloric restriction mimicked by 2DG protects retinas from NMDA excitotoxicity.

1Departments of Ophthalmology, Jules Stein Eye Institute

5Neuroscience, University of California Los Angeles School of Medicine, Room B146, 100 Stein Plaza, Los Angeles, CA 90095-7000, USA

2Department of Ophthalmology, Mackay Memorial Hospital, Taipei, Taiwan

3Mackay Junior College of Nursing, Taipei, Taiwan,ROC

4Department of Ophthalmology, Oita Medical University, Oita, Japan

CACorresponding Author: kwong@jsei.ucla.edu

Received 14 July 2003; accepted 3 October 2003

© 2003 Lippincott Williams & Wilkins, Inc.