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Distribution of PDE4A and Go immunoreactivity in the accessory olfactory system of the mouse

Lau, Yanny E.1; Cherry, James A.1,2

Chemical Neuroscience

Distribution of the cAMP-specific phosphodiesterase PDE4A was examined in the accessory olfactory system by immunohistochemistry. Adjacent sections through the vomeronasal organ (VNO) and accessory olfactory bulb (AOB) were alternately immunostained with antibodies against PDE4A or the G-protein α subunit G, which labels basal VNO neurons, in order to determine whether PDE4A occurs preferentially in one of two segregated VNO pathways. We found that PDE4A strongly labeled apical VNO neurons and rostral AOB glomeruli. There was virtually no overlap in G and PDE4A staining, and there were no regions of the VNO neuroepithelium or AOB glomeruli not labeled by either antibody. These results identify a potential member of the pheromone transduction cascade in apical neurons, and provide further evidence that the VNO consists of functionally distinct pathways.

1Department of Psychology and Laboratory of Molecular Neurobiology and Behavior, 64 Cummington Street, Boston University, Boston, MA 02215, USA

2Corresponding Author: James A. Cherry

Acknowledgements: This study was supported by NIH grants DC03019 and MH59200. We thank Dr M. J. Baum for a critical reading of the manuscript.

Received 1 September 1999; accepted 19 October 1999

© 2000 Lippincott Williams & Wilkins, Inc.