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Branchiomotor activities in mouse embryo

Abadie, Véronique1,2,3; Champagnat, Jean1; Fortin, Gilles1

Developmental Neuroscience

Using a novel isolated hindbrain in vitro preparation, we demonstrate that, in the mouse, branchiomotor activities from trigeminal, facial, glossopharyngeal and vagal nerves start during segmentation, a crucial and conserved period of hindbrain embryogenesis. At embryonic day (E) 10.5, branchiomotor nerves are independently active in bursts, become coactive at a low frequency (about 0.5 min−1 at E12.5, before high frequency (about 15 min−1) fetal breathing starts at E14.5. Comparison with observations in chick reveals a transient episodic rhythmic pattern highly similar in mouse at E13.5 and chick at E7. This pattern is proposed as a marker identifying a phylotypic stage during the development of hindbrain neuronal networks in vertebrates.

1Biologie Fonctionnelle du Neurone, Institut Alfred Fessard, CNRS, 1 avenue de la Terrasse, 91198 Gif Sur Yvette

2Department of Paediatrics, Hôpital Necker-Enfants Malades, 149 rue de Sèvres 75345 Paris cedex 15, France

3Corresponding Author: Véronique Abadie

Acknowledgements: This work was supported by the CNRS and a Human Frontier Science Program RGO101/1997-B to J.C, and by the Foundation pour la Recherche Médicale. We are thankful to Alan Strickland for his help in preparing this manuscript. V. A. was supported by the AP-HP/CNRS program PRA 99.005.

Received 22 September 1999; accepted 3 November 1999

© 2000 Lippincott Williams & Wilkins, Inc.