Objective: Cardiovascular disease (CVD) is a leading cause of morbidity and mortality in postmenopausal women. Danshen, the dried root of Salvia miltiorrhiza Bunge, has been used clinically in China to treat CVD and dyslipidemia in postmenopausal women, and its major active ingredients have been found to have an estrogenic effect. The aim of this study was to elucidate the underlying mechanism of danshen’s protective effects on vascular function in an ovariectomized (OVX) hyperlipidemic rat model.
Methods: Thirty-five 6-month-old female Sprague-Dawley rats were randomly divided into five groups: sham-operated rats with low-fat control diet + vehicle, sham-operated rats with high-fat diet (HFD) + vehicle, OVX rats with HFD + vehicle, OVX rats with HFD + 17β-estradiol (1 mg kg−1 d−1, PO), and OVX rats with HFD + danshen aqueous extract (600 mg kg−1 d−1, PO). After 12 weeks of treatment, gains in body weight and serum lipid profile levels in rats were measured and histological examination of livers was carried out. Vascular function was evaluated by measuring relaxation responses. Molecular mechanisms were also analyzed in isolated aorta.
Results: Treatment with danshen aqueous extract reduced body weight gain, improved serum lipid profiles, and prevented formation of fatty liver induced by HFD and OVX. In addition, danshen could increase endothelial-dependent vasorelaxation and displayed vasoprotection in OVX rats fed with HFD, primarily by stimulating nitric oxide (NO) production, up-regulating the mRNA expression of endothelial NO synthase, and down-regulating the mRNA expression of tumor necrosis factor α, intercellular cell adhesion molecule-1, and vascular cell adhesion molecule-1 in the isolated aortas.
Conclusions: We conclude for the first time that danshen aqueous extract could protect OVX rats fed with HFD from endothelial dysfunction. Its effect may be related to its abilities to normalize serum lipid profiles and enhance NO availability in the vascular system. Our findings indicate that danshen aqueous extract could be a promising natural supplement for postmenopausal women for preventing CVD.
Danshen aqueous extract could protect ovariectomized rats fed with high-fat diet from endothelial dysfunction.
From the 1College of Light Industry and Food, South China University of Technology, Guangzhou, PR China; 2Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical College, Guangzhou, PR China; 3State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, PR China; and 4Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR.
Received February 28, 2012; revised and accepted April 19, 2012.
Funding/support: This research was financially supported by the Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, and State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen.
Financial disclosure/conflicts of interest: None reported.
Address correspondence to: Shun-wan Chan, PhD, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR. E-mail: firstname.lastname@example.org