Institutional members access full text with Ovid®

Share this article on:

Polymorphisms in the estrogen synthesis and metabolism pathways and symptoms during the menopausal transition: observations from the Seattle Midlife Women's Health Study

Woods, Nancy F. PhD, RN1; Mitchell, Ellen Sullivan PhD, RN1; Tao, Yun MD1; Viernes, Hannah-Malia A. BS2; Stapleton, Patricia L. MS2; Farin, Federico M. MD2

doi: 10.1097/01.gme.0000227058.70903.9f
Articles

Objective: The purpose of this study was to determine whether polymorphisms in the estrogen synthesis and metabolism pathways are associated with women's vasomotor symptom experiences during the menopausal transition.

Design: In 2002, a subset of women enrolled in the Seattle Midlife Women's Health Study since 1990 (N = 174) provided a buccal smear for genotyping. Women were recruited by complete ascertainment of selected multiethnic neighborhoods in 1990. Participants were midlife women with a mean age of 53 years in 2005, well educated, employed, married, and represented a multiethnic population. Genotyping was done for the following polymorphisms: CYP1A1m2; CYP1B1*2 and CYP1B1*3; CYP17 5′UTR; CYP19 3′UTR; CYP19 (TTTA)n; including CYP19 7r and CYP19 7(r-3); CYP19 8r and CYP19 11r; and ESR1PvuII and ESR1XbaI. Women rated their vasomotor symptom severity in diaries on days 5, 6, and 7 of the menstrual cycle or on a constant date each month for women skipping periods. Menopausal transition stage was determined from daily menstrual calendars. First voided urine specimens provided several times each year were assayed for estrone glucuronide.

Results: Women with the CYP19 11r polymorphism reported more severe and frequent hot flashes during the middle and late menopausal transition stages and postmenopause and higher E1G levels during middle and late stages. None of the other polymorphisms studied were related to hot flashes or to estrone glucuronide levels.

Conclusions: These findings suggest a possible role for CYP19 polymorphisms in estrogen levels and in vasomotor symptoms during the menopausal transition that warrants further study in larger and more diverse populations of women.

Women with the CYP19 11r polymorphism reported more severe and frequent hot flashes during the middle and late menopausal transition stages as well as during postmenopause. These women also had higher urinary esterone levels during the middle and late stages than those without the polymorphism.

From the 1Department of Family and Child Nursing, School of Nursing and 2Department of Environmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, WA.

Received September 22, 2005; revised and accepted February 7, 2006.

Funded by grants NINR R01NR004141 and NINR P30NR04001 from the National Institute of Nursing Research and grant NIEHS P30ES07033 from the National Institute of Environmental Health Sciences.

Address correspondence to: Nancy F. Woods, PhD, RN, Department of Family and Child Nursing, University of Washington School of Nursing, T318, Health Sciences Building, Box 357260, Seattle, WA 98195-7260. E-mail: nfwoods@u.washington.edu.

©2006The North American Menopause Society