This document was originally developed by the WOCN Society's Clinical Practice Wound Subcommittee as a best practice document for clinicians.1 Its purpose is to provide clinicians with relevant information about the ankle brachial index (ABI) and a research-based protocol to use in performing the ABI to insure reliability and validity of the results.
WOCN Clinical Practice Wound Subcommittee, 2005
Updated/Revised: WOCN Wound Committee, 2010-2011
Original Publication Date: 2005
Introduction to Problems/Needs
Lower extremity arterial disease (LEAD) is a chronic, progressive disease. Risk factors for LEAD are advanced age, tobacco use, diabetes, dyslipidemia, hypertension, hyperhomocysteinemia, chronic renal insufficiency, family history of cardiovascular disease and African American ethnicity.2 Current data about prevalence and incidence of LEAD in the U.S. are limited. According to a hallmark U.S. study (N = 6979), 29% of individuals aged 70 years or older and 29% of individuals aged 50 through 69 years (that have a history of tobacco use or diabetes), had LEAD based on an ABI of less than 0.9.3 More recently, investigators in a population-based study in Sweden (N = 5080), reported the prevalence of LEAD was 18%.4
Approximately half of individuals with LEAD are undiagnosed because they are asymptomatic or have atypical symptoms and health care providers use unreliable methods to assess for LEAD such as pulse palpation or a history of claudication.2,5–7 It is recommended that health care providers use valid and reliable, non-invasive tests such as the ABI to detect LEAD.2,8–10
Definition of ABI
ABI is a noninvasive vascular screening test to identify large vessel, peripheral arterial disease by comparing systolic blood pressures in the ankle to the higher of the brachial systolic blood pressures, which is the best estimate of central systolic blood pressure.8,11–14 ABI is performed using a continuous wave Doppler, a sphygmomanometer and pressure cuffs to measure brachial and ankle systolic pressures.14,15 The use of pulse palpation or automated blood pressure devices to measure blood pressures for the ABI are not considered reliable.16,17
The ABI has high sensitivity and specificity and its accuracy to establish the diagnosis of LEAD has been well established.9,15 ABI is a ratio derived from dividing the higher of the ankle pressures (i.e., dorsalis pedis and posterior tibial) for each leg by the higher of the right and left arm's brachial systolic pressures.6 If blood flow is normal in the lower extremities, the pressure at the ankle should equal or be slightly higher than that in the arm with an ABI of 1.0 or more.
An ABI less than 0.9 indicates LEAD.2,8,9,11 If performed by an educated professional, using proper equipment and following a research-based procedure, the ABI obtained using a pocket Doppler is interchangeable with vascular laboratory tests to detect LEAD.8,18
Purpose of ABI
The purpose of the ABI is to support the diagnosis of vascular disease by providing an objective indicator of arterial perfusion to a lower extremity.
Limitations of ABI
* The ABI is an indirect examination that infers the anatomical location of an occlusion or stenosis. The exact location of the stenosis or occlusion cannot be determined by ABI alone.8
* The ABI might be elevated (>1.3) due to calcification of medial arteries at the ankle in patients with diabetes, renal failure and rheumatoid arthritis; and in such cases, other vascular tests should be performed.19–22
* Investigators in a study of 1,762 individuals, referred for a vascular evaluation, reported that the ABI was elevated in 8.4% and the prevalence of LEAD in those individuals was 62.2%.23
* Some individuals with arterial stenosis can experience claudication symptoms with activity and normal ankle pressures at rest, warranting referral for further vascular evaluation and testing.24
Indications for ABI
* Rule out LEAD in any patient with a lower extremity wound.2
* Establish diagnosis of arterial disease in patients with suspected LEAD9:
* Intermittent claudication.
* Over 70 years of age.
* Over 50 years of age with a history of tobacco use or diabetes.
* Determine adequate arterial blood flow in lower extremities prior to compression therapy, or wound debridement:
* If the ABI is less than 0.8, sustained, high compression (i.e., 30–40 mmHg at the ankle) is not recommended.2
* In mixed venous/arterial disease (i.e., ABI is > 0.5 to < 0.8), reduced compression levels (i.e., 23–30 mmHg) are advised.2,25 If the ABI is less than 0.5, compression should be avoided and the patient referred to a vascular surgeon for surgical evaluation and/or further testing.2
* Assess wound healing potential.
Contraindications for ABI15
* Excruciating pain in lower legs/feet.
* Deep vein thrombosis, which could lead to dislodgement of the thrombosis, where referral would be indicated for a duplex ultrasound test.
* Severe pain associated with lower extremity wound(s).
Guideline for Performing ABI
Before performing ABI, it is important to obtain a thorough history and physical. The Table addresses relevant factors in assessment and performing the ABI: history/physical findings, considerations, and decision to proceed with ABI. ABI Procedure
TABLE-a. Relevant Fa...Image Tools
Prepare Equipment and Supplies9,11,14
TABLE-b. Relevant Fa...Image Tools
TABLE-c. Relevant Fa...Image Tools
TABLE-d. Relevant Fa...Image Tools
1. Gather equipment and supplies for the ABI.
* Portable Doppler with 8-10 MHz probe.
* Use a 5 MHz probe if a large amount of edema is present at the ankle.
* Aneroid sphygmomanometer.
* Ultrasound transmission gel.
* Alcohol pads to clean the Doppler.
* Gauze, tissue or pads to remove transmission gel from patient's skin.
* Towels, sheets, or blankets to cover trunk and extremities.
* Paper and pen for recording test results; calculator.
* Inspect equipment for damage and check batteries if a battery-operated Doppler is used.
* Replace equipment if damaged or not properly calibrated.
2. Pressure cuffs for ankles and arms should be long enough to fully encircle the limb. The cuff bladder width should be 40% of the limb circumference and long enough to cover 80% of the arm circumference.26
* Typically, 12 cm wide cuffs are used for arms and 10 cm wide cuffs at the ankles.
* Extra large adult cuffs might be needed (14 cm).
Prepare Patient and Environment9,11,14
1. Inquire about recent use of tobacco, caffeine, alcohol; recent heavy activity, and presence of pain. (Note: When possible, advise patient to avoid stimulants or heavy exercise for an hour prior to the test.)
2. Perform the ABI in a quiet, warm environment to prevent vasconstriction of the arteries (21−23 + 1 °C).11
3. The best ABI results are obtained when the patient is relaxed, comfortable, and has an empty bladder.
4. Explain the procedure to the patient.
5. Remove socks, shoes, and tight clothing to permit placement of pressure cuffs and access to pulse sites by Doppler.
6. Place the patient in a flat, supine position. Place one small pillow behind the patient's head for comfort.
7. Prior to placement of the cuff, apply a protective barrier (e.g., plastic wrap) on the extremities if any wounds or alterations in skin integrity are present.27
8. Place pressure cuffs with the bottom of the cuff approximately 2-3 cm above the cubital fossa on the arms and malleolus at the ankle.11,26,28
* Cuffs should be wrapped without wrinkles and placed securely to prevent slipping and movement during the test.
9. Cover the trunk and extremities to prevent cooling.
10. Ensure the patient is comfortable and have the patient rest for a minimum of 10 minutes prior to the test to allow pressures to normalize. The authors of a major vascular technology textbook suggest 20 minutes rest prior to the ABI.14
Measure Brachial Pressures with Doppler9,11,14
1. After the rest period, measure the arm and ankle pressures.
2. The arm should be relaxed, supported and at heart level.
3. Palpate the brachial pulse to determine location to obtain an audible pulse.
4. Apply transmission gel over the pulse site.
5. Place the tip of the Doppler probe at a 45° angle pointed towards the patient's head until an audible pulse signal is obtained.
6. Inflate the pressure cuff 20-30 mmHg above the point where the pulse is no longer audible.11,15,26
7. Deflate the pressure cuff at a rate of 2-3 mmHg per second,26,29 noting the manometer reading at which the first pulse signal is heard and record that systolic value.
8. Cleanse/remove gel from pulse site.
9. Repeat the procedure to measure the pressure on the other arm.
10. If a pressure needs to be repeated, wait 1 minute before re-inflating the cuff.
11. Use the higher of the right or left arm's brachial systolic pressures to calculate the ABI for both legs.
Measure Ankle Pressures with Doppler9,11,14
Place the cuff on the patient's lower leg with the bottom of the cuff approximately 2-3 cm above the malleolus.
1. Prior to placing the cuff, apply a protective barrier (e.g., plastic wrap) on the extremity if any wounds or alterations in skin integrity are present.
2. Measure both dorsalis pedis and posterior tibial pulses in each leg.
3. Locate the pulses by palpation or with the Doppler probe.
4. Apply transmission gel to the pulse site.
5. Place the tip of the Doppler probe at a 45° angle pointed towards the patient's knee until an audible pulse signal is obtained.
6. Inflate the pressure cuff 20-30 mmHg above the point where the pulse is no longer audible
7. Deflate the cuff slowly at a rate of 2-3 mmHg per second, noting the manometer reading at which the first pulse signal is heard and record that systolic value.
8. Cleanse/remove gel from pulse site.
9. Repeat the procedure to measure pressures on the other ankle.
10. If a pressure needs to be repeated, wait one minute before re-inflating the cuff.
11. Use the higher of the ankle pressures of each leg to calculate the ABI for each leg.
Calculate the ABI9–11,27
1. Divide the higher of the dorsalis pedis or posterior tibial systolic pressure for each ankle by the higher of the right and left brachial pressures to obtain the ABI for each leg.
Equation (Uncited)Image Tools
2. Interpret and compare the ABI values from each leg.2
Table. No title avai...Image Tools
1. Describe the patient's tolerance of the procedure, any problems encountered in the test or inability to perform ABI.
2. Document all brachial and ankle pressures in the medical record. Note any differences between the extremities.
* If there is a 15-20 mmHg difference in the brachial pressures, this suggests subclavian stenosis.14
* A difference of 20-30 mmHg in pressures between ankles, suggests obstructive disease in the leg with the lower pressure.14
3. Document the ABI values and the interpretation of perfusion status.
4. Document any education provided to the patient/family and their understanding or response.
5. Notify the referring health care provider of any inconsistency in the ABI and clinical findings or inability to perform ABI.
6. Document any follow-up plans and referrals/communications to other health care providers.
7. Note: If a waveform is obtained with the procedure, it should be interpreted by a qualified clinician and a copy placed in the medical record.
Indications for Referral to a Vascular Surgeon for Further Evaluation/Testing2
1. New onset of LEAD.
2. ABI <0.9 in cases where an ulcer fails to improve in 2-4 weeks of proper treatment or patient has severe rest pain or intermittent claudication
3. Toe pressure <30 mmHg or TBI <0.6.
4. Borderline, severe, or critical ischemia.
5. Inconsistency between ABI and clinical complaints or observations (i.e., normal ABI and patient complains of intermittent claudication).
6. Inability to perform ABI.
7. Elevated ABI >1.3 warrants further vascular testing such as photoplethysmography, transcutaneous oxygen measures, segmental pressures, duplex ultrasound, magnetic resonance angiography, or computed tomography.2,6,9
Indications for Urgent Referral to a Vascular Surgeon or Emergency Room2
2. Wound infection or cellulitis in an ischemic limb.
3. Sudden onset of 6 Ps (i.e., pain, pulselessness, pallor, parathesia, paralysis, polar [coldness]), which indicates acute limb ischemia associated with a thrombosis.
Acute deep vein thrombosis (DVT): A thrombus or the formation of a blood clot that causes an outflow obstruction in the deep veins of the extremity. Veins distal to the obstruction become distended and venous pressure increases resulting in venous stasis. Signs and symptoms include pain, edema, erythema of the extremity and a positive Homan's sign.
Adult blood pressure cuff, large: Upper extremity, large, adult blood pressure cuff, which is appropriate for an arm circumference of 32.1-43.4 cm (12.6-17.1 in.). It has a length of 64.39 cm (25.35 in.) and a width of 17.02 cm (6.70 in.).
Arterial insufficiency: Lack of sufficient blood flow in arteries to extremities, which can be caused by cholesterol deposits (atherosclerosis), clots (emboli), or damaged, diseased, or weakened vessels.
Compression therapy: Application of sustained external pressure to the affected lower extremity to control edema and aid the return of venous blood to the heart. May be achieved by static compression (i.e., elastic and/or inelastic wraps, garments, or orthotics with single or multi-components/layers) or dynamic compression (i.e., intermittent pneumatic compression pumps).
Doppler scanning: Doppler velocity waveform analysis uses continuous-wave Doppler ultrasound to record arterial pulsations in various lower-extremity arteries.
Dorsalis pedis artery: The continuation of the anterior tibial artery of the lower leg. It starts at the ankle joint, divides into five branches, and supplies various muscles of the foot and toes. The dorsalis pedis pulse can be palpated on the mid-dorsum of the foot, between the first and second metatarsals.
Duplex ultrasound: Test combines traditional ultrasound that uses sound waves that bounce off blood vessels to create images with Doppler ultrasonography that examines how sound waves reflect off moving objects such as red blood cells.
Non-compressible blood vessels: The process in which vessels become hardened by the deposition of calcium salts in the tissues.
Pain scale: A means to measure the existence and intensity of pain. A standardized pain assessment instrument, such as the Wong-Baker Faces Pain Rating Scale or 0-10 Numeric Pain Intensity Scale.
Photoplethysmography (PPG): PPG determines blood flow by attaching a photosensor/transducer to the skin, which emits an infrared light that is reflected by the red blood cells in the vessels and is detected by the transducer. The amount of light reflected, corresponds to pulsatile changes and the tissue's blood volume. Toe pressure is obtained by attaching a PPG photosensor to the toe pad to record pulse changes and a small digit pressure cuff is placed at the base of the toe to measure the pressure.
Posterior tibial artery: One of the parts of the popliteal artery of the leg. It divides into eight branches, which supply blood to different muscles of the lower leg, foot, and toes. It is situated midway between the medial malleolus and the medial process of the calcaneal tuberosity. The posterior tibial pulse can be palpated in the groove behind the medial malleolus.
Purpura: A disorder with bleeding beneath the skin or mucous membranes. It causes black and blue spots (ecchymoses) or pinpoint bleeding.
Toe brachial index (TBI): TBI is a noninvasive test of arterial perfusion of the lower extremity that is obtained by comparing the systolic pressure in the great toe or second toe (if great toe is absent) to the higher of the right or left arm's systolic pressures. The TBI is a ratio derived by dividing the toe pressure by the arm pressure.
Transcutaneous oxygen measurement: A test to determine the oxygen tension in the skin by placement of a sensor that measures the oxygen pressure at a localized area on the surface of the skin.
Venous insufficiency: Failure of the valves of the veins to function that leads to decreased return of venous blood from the legs to the trunk of the body; may produce edema.
1. Wound, Ostomy and Continence Nurses Society. Ankle Brachial Index: Best Practice for Clinicians. Mt. Laurel, NJ: Author; 2005.
2. Wound, Ostomy and Continence Nurses Society. Guideline for Management of Wounds in Patients with Lower-Extremity Arterial Disease. Mt. Laurel, NJ: Author; 2008.
3. Hirsch A, Criqui M, Treat-Jacobson D, et al. Peripheral arterial disease detection, awareness, and treatment in primary care. J Am Med Assoc. 2001;286:1317–1324.
4. Sigvant B, Wiberg-Hedman K, Bergqvist D, et al. A population-based study of peripheral arterial disease prevalence with special focus on critical limb ischemia and sex differences. J Vascul Surg. 2007;45:1185–1191.
5. Beckman J, Jaff M, Creager M. The United States preventive services task force recommendation statement on screening for peripheral arterial disease. More harm than benefit? Circulation. 2006;114–866.
6. Bonham PA, Kelechi T, Mueller M, Robison J. Are toe pressures measured by a portable photoplethysmograph equivalent to standard laboratory tests? JWOCN. 2010;37:475–486.
7. Mourad J, Cacoub P, Collet J, et al. Screening of unrecognized peripheral arterial disease (PAD) using ankle-brachial index in high cardiovascular risk patients free from symptomatic PAD. J Vascul Surg. 2009;50:572–580.
8. Bonham PA, Cappuccio M, Hulsey T, et al. Are ankle and toe brachial indices (ABI-TBI) obtained by a pocket Doppler interchangeable with those obtained by standard laboratory equipment? JWOCN. 2007;34:35–44.
9. Hirsch AT, Haskal ZJ, Hertzer NR, et al. ACC/AHA 2005 Practice Guidelines for the management of patients witperipheral arterial disease (lower extremity, renal, mesenteric, and abdominal aortic): a collaborative report from the American Association for Vascular Surgery/Society for Vascular Surgery, Society for Cardiovascular Angiography and Interventions, Society for Vascular Medicine and Biology, Society of Interventional Radiology, and the ACC/AHA Task Force on Practice Guidelines (Writing Committee to Develop Guidelines for the Management of Patients With Peripheral Arterial Disease): Endorsed by the American Association of Cardiovascular and Pulmonary Rehabilitation; National Heart, Lung, and Blood Institute; Society for Vascular Nursing; TransAtlantic Inter-Society Consensus; and Vascular Disease Foundation. Circulation. 2006;113(11):e463–654.
10. Hopf HW, Ueno C, Aslam R, et al. Guidelines for the treatment of arterial insuffiency ulcers. Wound Repair Regenerat. 2006;14:693–710.
11. Bonham PA. Get the LEAD Out: noninvasive assessment for lower extremity arterial disease using ankle brachial index and toe brachial index measurements. JWOCN. 2006;33:30–41.
12. Criqui MH, Fronek A, Barrett-Conner E, Klauber MR, Babriel S, Goodman D. The prevalence of peripheral arterial disease in a defined population. Circulation. 1985;71:510–517.
13. Criqui MH, Browner D, Fronek A, et al. Peripheral arterial disease in large vessels is epidemiologically distinct from small vessel disease. Am J Epidemiol. 1989;129:1110–1119.
14. Rumwell C, McPharlin M. Vascular Technology: An Illustrated Review. 4th ed. Pasadena, CA: Davies Publishing, Inc.; 2009.
15. Grenon SM, Gagnon J, Hslang J. Ankle-brachial index for assessment of peripheral arterial disease. N Engl J Med. 2009;361:e40.
16. Aboyans V, Lacroix P, Doucet S, Preux P, Criqui MH, Laskar M. Diagnosis of peripheral arterial disease in general practice: can the ankle-brachial index be measured either by pulse palpation or an automatic blood pressure device? Int J Clin Pract. 2008;62:1001–1007.
17. Ena J, Lozano T, Verdu G, Argente C, Gonzalez V. Accuracy of ankle-brachial index obtained by automated blood pressure measuring devices in patients with diabetes mellitus. Diab Res Clin Pract. 2011;92:329–336.
18. Saskia PA, Kruidenier LM, Rouwet EV, et al. Pocket Doppler and vascular laboratory equipment yield comparable results for ankle brachial index measurement. BMC Cardiovascular Disorders. 2008;8:25. http://www.biomedcentral.com/1471-2261/8/26
19. Brooks B, Dean R, Patel S, Wu B, Molyneaus L, Yue K. TBI or not TBI: That is the question. Is it better to measure toe pressure than ankle pressure in diabetic patients? Diabet Med. 2001;18:528–532.
20. del Rincon I, Haas R, Pogosian S, Escalante A. Lower limb arterial incompressibility and obstruction in rheumatoid arthritis. Ann Rheumat Dis. 2005;64:425–432.
21. O'Hare A, Glidden D, Fox C, Hsu C. High prevalence of peripheral arterial disease in persons with renal insufficiency. Circulation. 2004;109:320–323.
22. Silvestro A, Diehm N, Savolainen H, et al. Falsely high ankle brachial index predicts major amputation in critical limb ischemia. Vascul Med. 2006;11:69–74.
23. Suominen V, Rantanen T, Venermo M, Saarinen J, Salenius J. Prevalence and risk factors of PAD among patients with elevated ABI. Eur J Vascul Endovas Surg. 2008;35:709–714.
24. Stein R, Hrijac I, Halperin JL, Gustavson SM, Teodorescu V, Olin J. Limitation of the resting ankle-brachial index in symptomatic patients with peripheral arterial disease. Vascul Med. 2006;11:29–33.
25. Wound, Ostomy and Continence Nurses Society. Guideline for Management of Wounds in Patients with Lower-Extremity Venous Disease. Mt. Laurel, NJ: Author; 2011.
26. Pickering TG, Hall JE, Appel LJ, Falkner BE, Graves MN, Rocella EJ. Recommendations for blood pressure measurements in humans and experimental animals: part I: blood pressure measurement in humans: A statement for professionals from the subcommittee of professional and public education of the American Heart Association Council on High Blood Pressure Research. Hypertension. 2005;45:142–161.
27. Vowden KR, Vowden P. Doppler and the ABPI: how good is our understanding? J Wound Care. 2001;10:197–201.
28. Augustine MJ, Eagleton KJ, Graham DH, Story S, Sullivan W, Koontz C. Accuracy of the ankle brachial pressure measurement by Physical Therapists and Physical Therapy students. Cardiopul Phys Ther J. 2000;11:99–104.
29. McDermott MM, Greenland P, Liu K, et al. The ankle brachial index is associated with leg function and physical activity: the walking and leg circulation study. Ann Intern Med. 2002;136:873–883.
30. Faglia E, Caravaggi C, Marchetti R, et al. Screening for peripheral arterial disease by means of the ankle-brachial index in newly diagnosed type 2 diabetic patients. Diabet Med. 2005;22:1310–1314.
31. Carter S, Tate R. The value of toe pulse waves in determination of risks for limb amputation and death in patients with peripheral arterial disease and skin ulcers or gangrene. J Vascul Surg. 2001;33:708–714.
32. de Graaf J, Ubbink D, Legemate D, Tijssen J, Jacobs M. Evaluation of toe pressure and transcutaneous oxygen measures in management of chronic leg ischemia: a diagnostic randomized clinical trial. J Vascul Surg. 2003;38:528–534.
33. Norgren L, Hiatt W, Dormandy J, Nehler M, Harris K, Fowkes F. Inter-Society Consensus for the management of peripheral arterial disease (TASCII). J Vascul Surg. 2007;45(Suppl S):S5A–S67A.
34. Wutschert R, Bounamequs H. Determination of amputation level in ischemic limbs. Diabet Care. 1997;20:1315–1318.
35. Wound, Ostomy and Continence Nurses Society. Toe Brachial Index: Best Practice for Clinicians. Mt. Laurel, NJ: Author; 2009.
36. Kline JA. Chapter 60. Thromboembolism. In: Tintinalli JE, Stapczynski JS, Cline DM, Ma OJ, Cydulka RK, Meckler GD. eds. Tintinalli's Emergency Medicine: A Comprehensive Study Guide. 2011:7e. http://www.accessmedicine.com/content.aspx?aID=6355989
. Accessed September 23, 2011.
38. Krasner DL. Wound Pain: Impact and assessment. In: Bryant R, Nix D. eds. Acute & Chronic Wounds. 4th ed. St. Louis, MO: Mosby; 2012:368–379.
39. Allison MA, Aboyans V, Granston T, et al. The relevance of different methods of calculating the ankle brachial index: the multi-ethnic study of atherosclerosis. Am J Epidemiol. 2010;171368–171376.
40. Carmel J. Venous Ulcers. In: Bryant R, Nix D. eds. Acute & Chronic Wounds. 4th ed. St. Louis, MO: Mosby; 2012:194–213.
41. Bjellerup M. Does dorsal pedal pulse palpation predict hand-held Doppler measurement of ankle-brachial index in leg ulcer patients? Wounds. 2003;15:237–240.
42. Collins T, Suarez-Almazor M, Petersen N. An absent pulse is not sensitive for the early detection of peripheral arterial disease. Fam Med. 2006;38:38–42.
43. Kazmers A, Koski ME, Groehn H, et al. Assessment of non-invasive lower extremity arterial testing versus pulse exam. Am Surg. 1996;62:315–319.
44. Lundkin M, Wikson J, Perakyla T, et al. Distal pulse palpation: is it reliable? World J Surg. 1999;23:252.
45. Magee T, Stanley P, Mufti R, Simpson L, Campbell W. Should we palpate foot pulses? Ann Royal Coll Surg Engl. 1992;74:166–168.
46. Moffiatt C, Ohare B. Ankle pulses are not sufficient to detect impaired arterial circulation in patients with leg ulcers. J Wound Care. 1995;4:134–138.
47. Carter S, Lezack J. Digital systolic pressures in the lower limb in arterial disease. Circulation. 1971;43:905–914.
48. Doughty D. Arterial Ulcers. In: Bryant R, Nix D. eds. Acute & Chronic Wounds. 4th ed. St. Louis, MO: Mosby; 2012:178–193.
49. Nicoloff A, Taylor L, Sexton G, et al. Relationship between site of initial symptoms and subsequent progression of disease in prospective study of atherosclerosis progression in patients receiving long-term treatment for symptomatic peripheral arterial disease. J Vascul Surg. 2002;35:38–47.
50. Driver VR, LeBretton JM, Landowski MA, Madsen JL. Neuropathic Wounds: The diabetic wound. In: Bryant R, Nix D, eds. Acute & Chronic Wounds. 4th ed. St. Louis, MO: Mosby; 2012:225–248.
51. Wound, Ostomy and Continence Nurses Society. Guideline for Management of Wounds in Patients with Lower-Extremity Neuropathic Disease. Mt. Laurel, NJ: Author; 2004.
52. Kavros A, Bubnik-Sttosek K. Ankle-brachial index screening for peripheral artery disease in asymptomatic patients between 50 and 70 years of age. J Int Med Res. 2009;37:1611–1619.